MYC amplification in multiple marker chromosomes and EZH2 microdeletion in a man with acute myeloid leukemia

Zhifu Xiang, Al Ola Abdallah, Rangaswamy Govindarajan, Paulette Mehta, Peter D. Emanuel, Peter Papenhausen, Steven A. Schichman

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The role of MYC and EZH2 in acute myeloid leukemia (AML) pathogenesis is poorly understood. Herein we present a case of AML with MYC amplification in marker chromosomes and a microdeletion of chromosome 7 below cytogenetic resolution. The karyotype of the patient's bone marrow aspirate showed three to five marker chromosomes in all dividing cells without other structural or numerical chromosomal abnormalities. Analysis by fluorescence in situ hybridization (FISH) with a probe specific for the human MYC gene revealed amplification of the oncogene localized to the marker chromosomes. Using whole genome single nucleotide polymorphism (SNP) microarray analysis, an approximately 4.4Mb amplicon containing the MYC gene was identified with an estimated amplification of about 30 copies per leukemic cell and, thus, an average of about 8 copies per marker chromosome. A 6.4Mb hemizygous microdeletion of chromosome 7 within band q36.1 was also found by SNP microarray analysis in a cellular-equivalent dosage of 50%. The microdeletion spans multiple genes, including EZH2, a gene with well-known cancer association. No mutation was found in the remaining EZH2 allele by next generation gene sequencing. The combination of MYC amplification and EZH2 deletion, which has not been described previously in AML, may suggest a synergistic role of the two oncogenes in the pathogenesis of the patient's acute leukemia.

Original languageEnglish
Pages (from-to)96-100
Number of pages5
JournalCancer Genetics
Volume208
Issue number3
DOIs
StatePublished - Mar 1 2015
Externally publishedYes

Keywords

  • Acute myeloid leukemia
  • EZH2
  • Genetic abnormality
  • MYC
  • Marker chromosomes

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    Xiang, Z., Abdallah, A. O., Govindarajan, R., Mehta, P., Emanuel, P. D., Papenhausen, P., & Schichman, S. A. (2015). MYC amplification in multiple marker chromosomes and EZH2 microdeletion in a man with acute myeloid leukemia. Cancer Genetics, 208(3), 96-100. https://doi.org/10.1016/j.cancergen.2015.01.010