TY - JOUR
T1 - Mutations in the cytomegalovirus UL97 gene associated with ganciclovir- resistant retinitis
AU - Liu, Weiqun
AU - Kuppermann, Baruch D.
AU - Martin, Daniel F.
AU - Wolitz, Richard A.
AU - Margolis, Todd P.
N1 - Funding Information:
Received 28 July 1997; revised 2 December 1997. Presented in part: annual meeting of the Association for Research in Vision and Ophthalmology (abstract 3071), Fort Lauderdale, Florida, April 1996. Human experimentation guidelines of the authors’ institutions were followed in the conduct of this study. Grant support: Universitywide AIDS Research Program, Oakland, California (R96-SF-153); NIH (EY-02162); Lew Wasserman Merit Award from Research to Prevent Blindness, New York, New York (to T.P.M.). Reprints or correspondence: Dr. Todd P. Margolis, F. I. Proctor Foundation, Box 0944, UCSF Medical Center, San Francisco, CA 94143-0944 (tpms@ itsa.ucsf.edu).
PY - 1998
Y1 - 1998
N2 - Vitreous from patients with cytomegalovirus (CMV) retinitis was studied in order to identify mutations in the CMV UL97 gene associated with clinical resistance to ganciclovir. Point mutations known to confer resistance (V460, I460, V594, and S595) were found in 6 of 11 study eyes. Rapid genetic screening by restriction enzyme analysis of vital DNA amplified directly from the vitreous was as effective as conventional sequencing in detecting these mutations. Repeat biopsy of 3 eyes revealed no change in the UL97 genotype. The UL97 genotype differed between eyes in 2 of 3 patients with bilateral, clinically resistant CMV retinitis. In summary, resistance mutations of the CMV UL97 gene are found in the vitreous of some, but not all, eyes with CMV retinitis that have not responded to ganciclovir therapy. These mutations can differ between eyes in patients with bilateral disease and can be rapidly detected using restriction digest analysis of polymerase chain reaction- amplified viral DNA.
AB - Vitreous from patients with cytomegalovirus (CMV) retinitis was studied in order to identify mutations in the CMV UL97 gene associated with clinical resistance to ganciclovir. Point mutations known to confer resistance (V460, I460, V594, and S595) were found in 6 of 11 study eyes. Rapid genetic screening by restriction enzyme analysis of vital DNA amplified directly from the vitreous was as effective as conventional sequencing in detecting these mutations. Repeat biopsy of 3 eyes revealed no change in the UL97 genotype. The UL97 genotype differed between eyes in 2 of 3 patients with bilateral, clinically resistant CMV retinitis. In summary, resistance mutations of the CMV UL97 gene are found in the vitreous of some, but not all, eyes with CMV retinitis that have not responded to ganciclovir therapy. These mutations can differ between eyes in patients with bilateral disease and can be rapidly detected using restriction digest analysis of polymerase chain reaction- amplified viral DNA.
UR - http://www.scopus.com/inward/record.url?scp=0031900985&partnerID=8YFLogxK
U2 - 10.1086/515293
DO - 10.1086/515293
M3 - Article
C2 - 9593000
AN - SCOPUS:0031900985
SN - 0022-1899
VL - 177
SP - 1176
EP - 1181
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -