Mutations in Saccharomyces cerevisiae gene SIR2 can have differential effects on in vivo silencing phenotypes and in vitro histone deacetylation activity

Christopher M. Armstrong, Matt Kaeberlein, Shin Ichiro Imai, Leonard Guarente

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

The yeast SIR2 gene and many of its homologs have been identified as NAD+-dependent histone deacetylases. To get a broader view of the relationship between the histone deacetylase activity of Sir2p and its in vivo functions we have mutated eight highly conserved residues in the core domain of SIR2. These mutations have a range of effects on the ability of Sir2p to deacetylate histones in vitro and to silence genes at the telomeres and HM loci. Interestingly, there is not a direct correlation between the in vitro and in vivo effects in some of these mutations. We also show that the histone deacetylase activity of Sir2p is necessary for the proper localiztion of the SIR complex to the telomeres.

Original languageEnglish
Pages (from-to)1427-1438
Number of pages12
JournalMolecular biology of the cell
Volume13
Issue number4
DOIs
StatePublished - 2002

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