Mutations in gld-1, a female germ cell-specific tumor suppressor gene in Caenorhabditis elegans, affect a conserved domain also found in Src- associated protein Sam68

A. R. Jones, T. Schedl

Research output: Contribution to journalArticlepeer-review

217 Scopus citations

Abstract

The gld-1 gene of Caenorhabditis elegans is a germ-line-specific tumor suppressor gene that is essential for oogenesis. We have cloned the gld-1 gene and find that it encodes two proteins that differ by 3 amino acids. The predicted proteins contain a -170-amino-acid region that we term the GSG domain (GRP33/Sam68/GLD-1), on the basis of significant similarity between GLD-1, GRP33 from shrimp, and the Src-associated protein Sam68 from mouse (also described as GAPap62 from humans). A conserved structural motif called the KH domain is found within the larger GSG domain, suggesting a biochemical function for GLD-1 protein in binding RNA. The importance of the GSG domain to the function of gld-1 in vivo is revealed by mutations that affect 5 different conserved GSG domain residues. These include missense mutations in an absolutely conserved residue of the KH domain that eliminate the tumor suppressor function of gld-1.

Original languageEnglish
Pages (from-to)1491-1504
Number of pages14
JournalGenes and Development
Volume9
Issue number12
DOIs
StatePublished - 1995

Keywords

  • C. elegans
  • GSG domain
  • KH domain
  • germ-line development
  • tumor suppressor

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