TY - JOUR
T1 - Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses
AU - Lim, Yi Wen
AU - Mansfeld, Ben N.
AU - Schläpfer, Pascal
AU - Gilbert, Kerrigan B.
AU - Narayanan, Narayanan N.
AU - Qi, Weihong
AU - Wang, Qi
AU - Zhong, Zhenhui
AU - Boyher, Adam
AU - Gehan, Jackson
AU - Beyene, Getu
AU - Lin, Zuh Jyh Daniel
AU - Esuma, Williams
AU - Feng, Suhua
AU - Chanez, Christelle
AU - Eggenberger, Nadine
AU - Adiga, Gerald
AU - Alicai, Titus
AU - Jacobsen, Steven E.
AU - Taylor, Nigel J.
AU - Gruissem, Wilhelm
AU - Bart, Rebecca S.
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Cassava mosaic disease (CMD) suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to cassava mosaic geminiviruses. It has been reported that CMD2-type landraces lose resistance after regeneration through de novo morphogenesis. As full genome bisulfite sequencing failed to uncover an epigenetic mechanism for this loss of resistance, whole genome sequencing and genetic variant analysis was performed and the CMD2 locus was fine-mapped to a 190 kilobase interval. Collectively, these data indicate that CMD2-type resistance is caused by a nonsynonymous, single nucleotide polymorphism in DNA polymerase δ subunit 1 (MePOLD1) located within this region. Virus-induced gene silencing of MePOLD1 in a CMD-susceptible cassava variety produced a recovery phenotype typical of CMD2-type resistance. Analysis of other CMD2-type cassava varieties identified additional candidate resistance alleles within MePOLD1. Genetic variation of MePOLD1, therefore, could represent an important genetic resource for resistance breeding and/or genome editing, and elucidating mechanisms of resistance to geminiviruses.
AB - Cassava mosaic disease (CMD) suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to cassava mosaic geminiviruses. It has been reported that CMD2-type landraces lose resistance after regeneration through de novo morphogenesis. As full genome bisulfite sequencing failed to uncover an epigenetic mechanism for this loss of resistance, whole genome sequencing and genetic variant analysis was performed and the CMD2 locus was fine-mapped to a 190 kilobase interval. Collectively, these data indicate that CMD2-type resistance is caused by a nonsynonymous, single nucleotide polymorphism in DNA polymerase δ subunit 1 (MePOLD1) located within this region. Virus-induced gene silencing of MePOLD1 in a CMD-susceptible cassava variety produced a recovery phenotype typical of CMD2-type resistance. Analysis of other CMD2-type cassava varieties identified additional candidate resistance alleles within MePOLD1. Genetic variation of MePOLD1, therefore, could represent an important genetic resource for resistance breeding and/or genome editing, and elucidating mechanisms of resistance to geminiviruses.
UR - http://www.scopus.com/inward/record.url?scp=85133638274&partnerID=8YFLogxK
U2 - 10.1038/s41467-022-31414-0
DO - 10.1038/s41467-022-31414-0
M3 - Article
C2 - 35798722
AN - SCOPUS:85133638274
SN - 2041-1723
VL - 13
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 3933
ER -