Mutations along a TET2 active site scaffold stall oxidation at 5-hydroxymethylcytosine

Monica Yun Liu, Hedieh Torabifard, Daniel J. Crawford, Jamie E. DeNizio, Xing Jun Cao, Benjamin A. Garcia, G. Andrés Cisneros, Rahul M. Kohli

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Ten-eleven translocation (TET) enzymes catalyze stepwise oxidation of 5-methylcytosine (mC) to yield 5-hydroxymethylcytosine (hmC) and the rarer bases 5-formylcytosine (fC) and 5-carboxylcytosine (caC). Stepwise oxidation obscures how each individual base forms and functions in epigenetic regulation, and prompts the question of whether TET enzymes primarily serve to generate hmC or are adapted to produce fC and caC as well. By mutating a single, conserved active site residue in human TET2, Thr1372, we uncovered enzyme variants that permit oxidation to hmC but largely eliminate fC and caC. Biochemical analyses, combined with molecular dynamics simulations, elucidated an active site scaffold that is required for wild-Type (WT) stepwise oxidation and that, when perturbed, explains the mutants' hmC-stalling phenotype. Our results suggest that the TET2 active site is shaped to enable higher-order oxidation and provide the first TET variants that could be used to probe the biological functions of hmC separately from fC and caC.

Original languageEnglish
Pages (from-to)181-187
Number of pages7
JournalNature Chemical Biology
Volume13
Issue number2
DOIs
StatePublished - Feb 1 2017

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