Mutations affecting development of the notochord in zebrafish

Derek L. Stemple, Lilianna Solnica-Krezel, Fried Zwartkruis, Stephan C.F. Neuhauss, Alexander F. Schier, Jarema Malicki, Didier Y.R. Stainier, Salim Abdelilah, Zehava Rangini, Elizabeth Mountcastle-Shah, Wolfgang Driever

Research output: Contribution to journalArticle

140 Scopus citations

Abstract

The notochord is critical for the normal development of vertebrate embryos. It serves both as the major skeletal element of the embryo and as a signaling source for the establishment of pattern within the neurectoderm, the paraxial mesoderm and other tissues. In a large-scale systematic screen of mutations affecting embryogenesis in zebrafish we identified 65 mutations that fall into 29 complementation groups, each leading to a defect in the formation and/or maintenance of the notochord. These mutations produce phenotypic abnormalities at numerous stages of notochord development, thereby establishing a phenotypic pathway, which in turn suggests a genetic pathway for the development of the notochord. Perturbations within adjacent tissues in mutant embryos further indicate the importance of notochord-derived signals for patterning within the embryo and suggest that these mutations will yield additional insight into the cues that regulate these patterning processes.

Original languageEnglish
Pages (from-to)117-128
Number of pages12
JournalDevelopment
Volume123
StatePublished - Dec 1 1996
Externally publishedYes

Keywords

  • Embryogenesis
  • Floor plate
  • Mesoderm
  • Notochord
  • Zebrafish

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    Stemple, D. L., Solnica-Krezel, L., Zwartkruis, F., Neuhauss, S. C. F., Schier, A. F., Malicki, J., Stainier, D. Y. R., Abdelilah, S., Rangini, Z., Mountcastle-Shah, E., & Driever, W. (1996). Mutations affecting development of the notochord in zebrafish. Development, 123, 117-128.