Mutations affecting cell fates and cellular rearrangements during gastrulation in zebrafish

Lilianna Solnica-Krezel, Derek L. Stemple, Eliza Mountcastle-Shah, Zehava Rangini, Stephan C.F. Neuhauss, Jarema Malicki, Alexander F. Schier, Didier Y.R. Stainier, Fried Zwartkruis, Salim Abdelilah, Wolfgang Driever

Research output: Contribution to journalArticlepeer-review

266 Scopus citations

Abstract

One of the major challenges of developmental biology is understanding the inductive and morphogenetic processes that shape the vertebrate embryo. In a large-scale genetic screen for zygotic effect, embryonic lethal mutations in zebrafish we have identified 25 mutations that affect specification of cell fates and/or cellular rearrangements during gastrulation. These mutations define at least 14 complementation groups, four of which correspond to previously identified genes. Phenotypic analysis of the ten novel loci revealed three groups of mutations causing distinct effects on cell fates in the gastrula. One group comprises mutations that lead to deficiencies in dorsal mesodermal fates and affect central nervous system patterning. Mutations from the second group affect formation of ventroposterior embryonic structures. We suggest that mutations in these two groups identify genes necessary for the formation, maintenance or function of the dorsal organizer and the ventral signaling pathway, respectively. Mutations in the third group affect primarily cellular rearrangements during gastrulation and have complex effects on cell fates in the embryo. This group, and to some extent mutations from the first two groups, affect the major morphogenetic processes, epiboly, convergence and extension, and tail morphogenesis. These mutations provide an approach to understanding the genetic control of gastrulation in vertebrates.

Original languageEnglish
Pages (from-to)67-80
Number of pages14
JournalDevelopment
Volume123
StatePublished - 1996

Keywords

  • Convergence
  • Dorsoventral polarity
  • Epiboly
  • Extension
  • Gastrulation
  • Organizer
  • Zebrafish

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