TY - JOUR
T1 - Murine FGFR-1 is required for early postimplantation growth and axial organization
AU - Deng, Chu Xia
AU - Wynshaw-Boris, Anthony
AU - Shen, Michael M.
AU - Daugherty, Cathie
AU - Ornitz, David M.
AU - Leder, Philip
PY - 1994/12/15
Y1 - 1994/12/15
N2 - We have explored the role of fibroblast growth factor receptor 1 (FGFR-1) in early embryonic development using three experimental systems: genetically deficient mice, in vitro blastocyst culture, and FGFR-1-deficient embryonic stem cells. Using these systems, we demonstrate that FGFR-1 is required for proper embryonic cell proliferation and for the correct axial organization of early postimplantation embryos but not for mesoderm formation. FGFR-1- deficient embryos display severe growth retardation both in vitro and in vivo and die prior to or during gastrulation. Although these mutants can form nonaxial tissues, such as the allantois, amnion, and yolk sac mesoderm, they display defective patterning of the primitive streak and other axial structures, and frequently exhibit truncations or disorganization of posterior embryonic regions. Such abnormalities are unlikely to be caused by intrinsic blocks in mesodermal differentiation, as FGFR-1-deficient ES cell lines form teratomas consisting of many mesodermal cell types.
AB - We have explored the role of fibroblast growth factor receptor 1 (FGFR-1) in early embryonic development using three experimental systems: genetically deficient mice, in vitro blastocyst culture, and FGFR-1-deficient embryonic stem cells. Using these systems, we demonstrate that FGFR-1 is required for proper embryonic cell proliferation and for the correct axial organization of early postimplantation embryos but not for mesoderm formation. FGFR-1- deficient embryos display severe growth retardation both in vitro and in vivo and die prior to or during gastrulation. Although these mutants can form nonaxial tissues, such as the allantois, amnion, and yolk sac mesoderm, they display defective patterning of the primitive streak and other axial structures, and frequently exhibit truncations or disorganization of posterior embryonic regions. Such abnormalities are unlikely to be caused by intrinsic blocks in mesodermal differentiation, as FGFR-1-deficient ES cell lines form teratomas consisting of many mesodermal cell types.
KW - Gene targeting
KW - gastrulation
KW - mesoderm induction
KW - pattern formation
UR - http://www.scopus.com/inward/record.url?scp=0028598814&partnerID=8YFLogxK
U2 - 10.1101/gad.8.24.3045
DO - 10.1101/gad.8.24.3045
M3 - Article
C2 - 8001823
AN - SCOPUS:0028598814
SN - 0890-9369
VL - 8
SP - 3045
EP - 3057
JO - Genes and Development
JF - Genes and Development
IS - 24
ER -