TY - JOUR
T1 - Murine aldo-keto reductase family 1 subfamily B
T2 - Identification of AKR1B8 as an ortholog of human AKR1B10
AU - Joshi, Amit
AU - Rajput, Sandeep
AU - Wang, Chun
AU - Ma, Jun
AU - Cao, Deliang
PY - 2010/12
Y1 - 2010/12
N2 - Aldo-keto reductase family 1 member B10 (AKR1B10), overexpressed in multiple human cancers, might be implicated in cancer development and progression via detoxifying cytotoxic carbonyls and regulating fatty acid synthesis. In the present study, we investigated the ortholog of AKR1B10 in mice, an ideal modeling organism greatly contributing to human disease investigations. In the mouse, there are three aldo-keto reductase family 1 subfamily B (AKR1B) members, i.e., AKR1B3, AKR1B7, and AKR1B8. Among them, AKR1B8 has the highest similarity to human AKR1B10 in terms of amino acid sequence, computer-modeled structures, substrate spectra and specificity, and tissue distribution. More importantly, similar to human AKR1B10, mouse AKR1B8 associates with murine acetyl-CoA carboxylase-a and mediates fatty acid synthesis in colon cancer cells. Taken together, our data suggest that murine AKR1B8 is the ortholog of human AKR1B10.
AB - Aldo-keto reductase family 1 member B10 (AKR1B10), overexpressed in multiple human cancers, might be implicated in cancer development and progression via detoxifying cytotoxic carbonyls and regulating fatty acid synthesis. In the present study, we investigated the ortholog of AKR1B10 in mice, an ideal modeling organism greatly contributing to human disease investigations. In the mouse, there are three aldo-keto reductase family 1 subfamily B (AKR1B) members, i.e., AKR1B3, AKR1B7, and AKR1B8. Among them, AKR1B8 has the highest similarity to human AKR1B10 in terms of amino acid sequence, computer-modeled structures, substrate spectra and specificity, and tissue distribution. More importantly, similar to human AKR1B10, mouse AKR1B8 associates with murine acetyl-CoA carboxylase-a and mediates fatty acid synthesis in colon cancer cells. Taken together, our data suggest that murine AKR1B8 is the ortholog of human AKR1B10.
KW - AKR1B8 knockdown
KW - Acetyl-CoA carboxylase-α
KW - Aldo-keto reductase family 1 member B10 (AKR1B10)
KW - Aldo-keto reductase family 1 member B8 (AKR1B8)
KW - Mouse ortholog
UR - https://www.scopus.com/pages/publications/79952523150
U2 - 10.1515/BC.2010.144
DO - 10.1515/BC.2010.144
M3 - Article
C2 - 21087085
AN - SCOPUS:79952523150
SN - 1431-6730
VL - 391
SP - 1371
EP - 1378
JO - Biological Chemistry
JF - Biological Chemistry
IS - 12
ER -