TY - JOUR
T1 - Murine AIDS superantigen reactivity of the T cells bearing Vβ5 T cell antigen receptor
AU - Kanagawa, O.
AU - Nussrallah, B. A.
AU - Wiebenga, M. E.
AU - Murphy, K. M.
AU - Morse, H. C.
AU - Carbone, F. R.
PY - 1992/7/1
Y1 - 1992/7/1
N2 - A B cell line, B6-1710, that expresses the defective virus known to induce murine AIDS stimulates a large fraction of nonprimed splenic T cells. Analysis of the T cell population responding to the B6-1710 for TCR Vβ-chain usage revealed that, in addition to the previously reported Vβ5-chain-positive T cells, T cells bearing Vβ11 and Vβ12 are also specifically enriched. We have established Vβ5+ T cell lines, clones, and hybridomas expressing identical TCR with different CD4/CD8 phenotypes and demonstrated that T cell reactivity to B6-1710 is, although not absolute, dependent on the presence of CD4 molecules. Further analysis of T cell hybridomas with known Jβ-chain usage revealed that Dβ-and Jβ-chain usage do not play crucial roles in T cell reactivity to B6-1710 B cells. However, T cell hybridomas derived from TCR-Vβ gene transgenic mice were found to be heterogeneous for their reactivity to B6-1710, suggesting that the Vα-chains associating with the transgenic Vβ-chain determine T cell responsiveness to B6-1710. These data clearly demonstrate that T cell reactivity to a murine AIDS virus expressing B cell line resembles that previously reported for Mls-like superantigens.
AB - A B cell line, B6-1710, that expresses the defective virus known to induce murine AIDS stimulates a large fraction of nonprimed splenic T cells. Analysis of the T cell population responding to the B6-1710 for TCR Vβ-chain usage revealed that, in addition to the previously reported Vβ5-chain-positive T cells, T cells bearing Vβ11 and Vβ12 are also specifically enriched. We have established Vβ5+ T cell lines, clones, and hybridomas expressing identical TCR with different CD4/CD8 phenotypes and demonstrated that T cell reactivity to B6-1710 is, although not absolute, dependent on the presence of CD4 molecules. Further analysis of T cell hybridomas with known Jβ-chain usage revealed that Dβ-and Jβ-chain usage do not play crucial roles in T cell reactivity to B6-1710 B cells. However, T cell hybridomas derived from TCR-Vβ gene transgenic mice were found to be heterogeneous for their reactivity to B6-1710, suggesting that the Vα-chains associating with the transgenic Vβ-chain determine T cell responsiveness to B6-1710. These data clearly demonstrate that T cell reactivity to a murine AIDS virus expressing B cell line resembles that previously reported for Mls-like superantigens.
UR - http://www.scopus.com/inward/record.url?scp=0026642425&partnerID=8YFLogxK
M3 - Article
C2 - 1351503
AN - SCOPUS:0026642425
SN - 0022-1767
VL - 149
SP - 9
EP - 16
JO - Journal of Immunology
JF - Journal of Immunology
IS - 1
ER -