Multispectral imaging of tissue absorption and scattering using spatial frequency domain imaging and a computed-tomography imaging spectrometer

Jessie R. Weber, David J. Cuccia, William R. Johnson, Gregory H. Bearman, Anthony J. Durkin, Mike Hsu, Alexander Lin, Devin K. Binder, Dan Wilson, Bruce J. Tromberg

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65 Scopus citations


We present an approach for rapidly and quantitatively mapping tissue absorption and scattering spectra in a wide-field, noncontact imaging geometry by combining multifrequency spatial frequency domain imaging (SFDI) with a computed-tomography imaging spectrometer (CTIS). SFDI overcomes the need to spatially scan a source, and is based on the projection and analysis of periodic structured illumination patterns. CTIS provides a throughput advantage by simultaneously diffracting multiple spectral images onto a single CCD chip to gather spectra at every pixel of the image, thus providing spatial and spectral information in a single snapshot. The spatial-spectral data set was acquired 30 times faster than with our wavelength-scanning liquid crystal tunable filter camera, even though it is not yet optimized for speed. Here we demonstrate that the combined SFDI-CTIS is capable of rapid, multispectral imaging of tissue absorption and scattering in a noncontact, nonscanning platform. The combined system was validated for 36 wavelengths between 650-1000 nm in tissue simulating phantoms over a range of tissue-like absorption and scattering properties. The average percent error for the range of absorption coefficients (μa) was less than 10 from 650-800 nm, and less than 20 from 800-1000 nm. The average percent error in reduced scattering coefficients (μs′) was less than 5 from 650-700 nm and less than 3 from 700-1000 nm. The SFDI-CTIS platform was applied to a mouse model of brain injury in order to demonstrate the utility of this approach in characterizing spatially and spectrally varying tissue optical properties.

Original languageEnglish
Article number011015
JournalJournal of biomedical optics
Issue number1
StatePublished - Jan 2011


  • spectrometry; brain imaging; absorption; scattering; optical properties; intrinsic signal


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