@inbook{a19d3a9a77454813abd393b57882fab1,
title = "Multiplex and quantitative pathogen detection with high-resolution capillary electrophoresis-based single-strand conformation polymorphism",
abstract = "Among the molecular diagnostic methods for bacteria-induced diseases, capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) combined with 16S rRNA gene-specific PCR has enormous potential because it can separate sequence variants using a simple procedure. However, conventional CE-SSCP systems have limited resolution and cannot separate most 16S rRNA gene-specific markers into separate peaks. A high-resolution CE-SSCP system that uses a poly(ethyleneoxide)-poly(propyleneoxide)- poly(ethyleneoxide) triblock copolymer matrix was recently developed and shown to effectively separate highly similar PCR products. In this report, a protocol for the detection of 12 pathogenic bacteria is provided. Pathogen markers were amplified by PCR using universal primers and separated by CE-SSCP; each marker peak was well separated at baseline and showed a characteristic mobility, allowing the easy identification of the pathogens.",
keywords = "16S rRNA gene, CE-SSCP, High-resolution, Pathogen detection, Polymer matrix",
author = "Hwang, {Hee Sung} and Shin, {Gi Won} and Boram Chung and Jeongkyeong Na and Jung, {Gyoo Yeol}",
year = "2013",
doi = "10.1007/978-1-62703-29-8_15",
language = "English",
isbn = "9781627030281",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "155--163",
booktitle = "Clinical Applications of Capillary Electrophoresis",
}