Multiple gabaa receptor α subunit mRNAs revealed by developmental and regional expression in rat, chicken and human brain

Pascale Montpied; Edward I. Ginns; Brian M. Martin; Deborah Steuer; Anne Marie O'Carroll; Stephen J. Lolait; Lawrence C. Mahan; Steven M. Paul

doi:10.1016/0014-5793(89)81623-0

Multiple gabaa receptor α subunit mRNAs revealed by developmental and regional expression in rat, chicken and human brain

Pascale Montpied, Edward I. Ginns, Brian M. Martin, Deborah Steuer, Anne Marie O'Carroll, Stephen J. Lolait, Lawrence C. Mahan, Steven M. Paul

Washington University School of Medicine

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

GABA_A receptor α subunit transcripts were detected by Northern analysis of rat, chicken and human brain mRNA using a series of ³² P-labelled antisense RNA probes derived from human α₁ subunit cDNAs. These α subunit mRNAs differ in their distribution among various brain regions in the rat and at least one species is detected primarily in fetal brain. GABA_A receptor α_a subunit probes encoding the putative extracellular domain detect at least five α subunit transcripts in rat brain, whereas probes encoding the putative intracellular domain detect only two mRNAs. These data suggest the presence in brain of multiple GABA_A receptor α subunits having homologous extracellular domains and whose expression is regionally and developmentally regulated. These α subunit transcripts may encode proteins that comprise GABA_A isoreceptors differing in their pharmacological and physiological properties.

Original language	English
Pages (from-to)	94-98
Number of pages	5
Journal	FEBS Letters
Volume	258
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(89)81623-0
State	Published - Nov 20 1989

Keywords

(Rat, Chicken, Human, Brain)
Developmental expression
GABA/benzodiazepine receptor
Isoreceptor
Multiple α subunit mRNA
Regional expression

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10.1016/0014-5793(89)81623-0

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@article{e59a6a5aed43497cbb6e6c793eaf50dd,

title = "Multiple gabaa receptor α subunit mRNAs revealed by developmental and regional expression in rat, chicken and human brain",

abstract = "GABAA receptor α subunit transcripts were detected by Northern analysis of rat, chicken and human brain mRNA using a series of 32 P-labelled antisense RNA probes derived from human α1 subunit cDNAs. These α subunit mRNAs differ in their distribution among various brain regions in the rat and at least one species is detected primarily in fetal brain. GABAA receptor αa subunit probes encoding the putative extracellular domain detect at least five α subunit transcripts in rat brain, whereas probes encoding the putative intracellular domain detect only two mRNAs. These data suggest the presence in brain of multiple GABAA receptor α subunits having homologous extracellular domains and whose expression is regionally and developmentally regulated. These α subunit transcripts may encode proteins that comprise GABAA isoreceptors differing in their pharmacological and physiological properties.",

keywords = "(Rat, Chicken, Human, Brain), Developmental expression, GABA/benzodiazepine receptor, Isoreceptor, Multiple α subunit mRNA, Regional expression",

author = "Pascale Montpied and Ginns, {Edward I.} and Martin, {Brian M.} and Deborah Steuer and O'Carroll, {Anne Marie} and Lolait, {Stephen J.} and Mahan, {Lawrence C.} and Paul, {Steven M.}",

note = "Funding Information: Acknowledgements: S.J.L. was supportedb y a C.J. Martin Fellowship from the National Health and Medical ResearchC ouncil of Australia. The authors thank MS Kay Kuhns for typing the manuscript.",

year = "1989",

month = nov,

day = "20",

doi = "10.1016/0014-5793(89)81623-0",

language = "English",

volume = "258",

pages = "94--98",

journal = "FEBS Letters",

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T1 - Multiple gabaa receptor α subunit mRNAs revealed by developmental and regional expression in rat, chicken and human brain

AU - Montpied, Pascale

AU - Ginns, Edward I.

AU - Martin, Brian M.

AU - Steuer, Deborah

AU - O'Carroll, Anne Marie

AU - Lolait, Stephen J.

AU - Mahan, Lawrence C.

AU - Paul, Steven M.

N1 - Funding Information: Acknowledgements: S.J.L. was supportedb y a C.J. Martin Fellowship from the National Health and Medical ResearchC ouncil of Australia. The authors thank MS Kay Kuhns for typing the manuscript.

PY - 1989/11/20

Y1 - 1989/11/20

N2 - GABAA receptor α subunit transcripts were detected by Northern analysis of rat, chicken and human brain mRNA using a series of 32 P-labelled antisense RNA probes derived from human α1 subunit cDNAs. These α subunit mRNAs differ in their distribution among various brain regions in the rat and at least one species is detected primarily in fetal brain. GABAA receptor αa subunit probes encoding the putative extracellular domain detect at least five α subunit transcripts in rat brain, whereas probes encoding the putative intracellular domain detect only two mRNAs. These data suggest the presence in brain of multiple GABAA receptor α subunits having homologous extracellular domains and whose expression is regionally and developmentally regulated. These α subunit transcripts may encode proteins that comprise GABAA isoreceptors differing in their pharmacological and physiological properties.

AB - GABAA receptor α subunit transcripts were detected by Northern analysis of rat, chicken and human brain mRNA using a series of 32 P-labelled antisense RNA probes derived from human α1 subunit cDNAs. These α subunit mRNAs differ in their distribution among various brain regions in the rat and at least one species is detected primarily in fetal brain. GABAA receptor αa subunit probes encoding the putative extracellular domain detect at least five α subunit transcripts in rat brain, whereas probes encoding the putative intracellular domain detect only two mRNAs. These data suggest the presence in brain of multiple GABAA receptor α subunits having homologous extracellular domains and whose expression is regionally and developmentally regulated. These α subunit transcripts may encode proteins that comprise GABAA isoreceptors differing in their pharmacological and physiological properties.

KW - (Rat, Chicken, Human, Brain)

KW - Developmental expression

KW - GABA/benzodiazepine receptor

KW - Isoreceptor

KW - Multiple α subunit mRNA

KW - Regional expression

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DO - 10.1016/0014-5793(89)81623-0

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C2 - 2556301

AN - SCOPUS:0024471983

SN - 0014-5793

VL - 258

SP - 94

EP - 98

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Multiple gabaa receptor α subunit mRNAs revealed by developmental and regional expression in rat, chicken and human brain

Abstract

Keywords

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