Multiple effects of N,N' dicyclohexyl carbodiimide on the beta-adrenergic receptor adenylate cyclase system in frog erythrocytes

L. J. Pike, R. J. Lefkowitz

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Treatment of frog erythrocytes with N,N'dicyclohexylcarbodiimide (DCCD) leads to a loss of catecholamine stimulated adenylate cyclase activity without any decrease in fluoride or PGE1 stimulated cyclase. However, the concentrations of the reagent which inhibit catecholamine sensitive adenylate cyclase activity are 10 fold lower than those which inhibit specific [3H]dihydroalprenolol ([3H]DHA) beta-adrenergic receptor binding. By contrast binding of the radiolabeled beta-adrenergic agonist [3H]hydroxybenzylisoproterenol ([3H]HBI) is considerably more sensitive than antagonist binding to the effects of DCCD. The data suggest that low concentrations of the reagent may modify the effector portion of the beta-adrenergic receptor leading to functional uncoupling of the beta-receptor adenylate cyclase system. At higher concentrations of the reagent the ligand binding site of the beta-receptor appears also to be altered.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalJournal of Cyclic Nucleotide Research
Volume4
Issue number1
StatePublished - Dec 1 1978
Externally publishedYes

Fingerprint Dive into the research topics of 'Multiple effects of N,N' dicyclohexyl carbodiimide on the beta-adrenergic receptor adenylate cyclase system in frog erythrocytes'. Together they form a unique fingerprint.

Cite this