Multiphoton microscopy with near infrared contrast agents

Siavash Yazdanfar; Chulmin Joo; Chun Zhan; Mikhail Y. Berezin; Walter J. Akers; Samuel Achilefu

doi:10.1117/1.3420209

Multiphoton microscopy with near infrared contrast agents

Siavash Yazdanfar
, Chulmin Joo
, Chun Zhan
, Mikhail Y. Berezin
, Walter J. Akers
, Samuel Achilefu

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

Original language	English
Article number	030505
Journal	Journal of biomedical optics
Volume	15
Issue number	3
DOIs	https://doi.org/10.1117/1.3420209
State	Published - May 2010

Keywords

Near-infrared imaging
Two-photon microscopy
Ultrafast fiber lasers

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10.1117/1.3420209

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title = "Multiphoton microscopy with near infrared contrast agents",

abstract = "While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.",

keywords = "Near-infrared imaging, Two-photon microscopy, Ultrafast fiber lasers",

author = "Siavash Yazdanfar and Chulmin Joo and Chun Zhan and Berezin, \{Mikhail Y.\} and Akers, \{Walter J.\} and Samuel Achilefu",

note = "Funding Information: This study was supported by a grant from the National Institutes of Health (R21/R33 CA12353701). We acknowledge S. Bhaumik, A. Sood, and J. DePuy for help in sample preparation, and P. Codella for performing linear spectroscopy.",

year = "2010",

month = may,

doi = "10.1117/1.3420209",

language = "English",

volume = "15",

journal = "Journal of biomedical optics",

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T1 - Multiphoton microscopy with near infrared contrast agents

AU - Yazdanfar, Siavash

AU - Joo, Chulmin

AU - Zhan, Chun

AU - Berezin, Mikhail Y.

AU - Akers, Walter J.

AU - Achilefu, Samuel

N1 - Funding Information: This study was supported by a grant from the National Institutes of Health (R21/R33 CA12353701). We acknowledge S. Bhaumik, A. Sood, and J. DePuy for help in sample preparation, and P. Codella for performing linear spectroscopy.

PY - 2010/5

Y1 - 2010/5

N2 - While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

AB - While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

KW - Near-infrared imaging

KW - Two-photon microscopy

KW - Ultrafast fiber lasers

UR - https://www.scopus.com/pages/publications/79956124717

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DO - 10.1117/1.3420209

M3 - Article

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AN - SCOPUS:79956124717

SN - 1083-3668

VL - 15

JO - Journal of biomedical optics

JF - Journal of biomedical optics

IS - 3

M1 - 030505

ER -

Multiphoton microscopy with near infrared contrast agents

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