29 Scopus citations


While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

Original languageEnglish
Article number030505
JournalJournal of biomedical optics
Issue number3
StatePublished - May 2010


  • Near-infrared imaging
  • Two-photon microscopy
  • Ultrafast fiber lasers


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