Multimodal optical and Gd-based nanoparticles for imaging in inflammatory arthritis

J. S. Kim, H. An, W. J. Rieter, D. Esserman, K. M.L. Taylor-Pashow, R. B. Sartor, W. Lin, W. Lin, Teresa K. Tarrant

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Objective: This report documents a multimodal nanoparticle (MNP) contrast agent, containing embedded luminophores and surface-immobilized gadolinium chelates, as a contrast agent of inflamed synovium in a collagen induced arthritis (CIA) model. Methods: DBA-U mice were immunized for CIA and imaged after disease onset by two independent modalities. After intravenous administration of MNP contrast, optical and magnetic resonance images were obtained and clinical disease was scored, which was followed by processing of hindlimbs for immunofluorescence and confocal microscopy. Results: We show a correlation between disease severity and MNP optical luminescence that is dose dependent. Immunofluorescence of hindlimb sections reveal that MNP-labeled cells are monocytes/macrophages within the inflamed synovium. Magnetic resonance (MR) relaxation time maps, which determine the quantitative measure of T1 and T2 values at each imaging voxel, demonstrated a decreasing T2 signal in actively inflamed joints that was more pronounced earlier rather than later during disease. Conclusions: MNPs containing surface-immobilized gadolinium chelates and embedded luminophores are potential dual-modality contrast agents in inflammatory arthritis and localize to monocytes/macrophages within inflamed synovium.

Original languageEnglish
Pages (from-to)580-586
Number of pages7
JournalClinical and Experimental Rheumatology
Volume27
Issue number4
StatePublished - Jul 1 2009
Externally publishedYes

Keywords

  • Arthritis
  • Diagnostic imaging
  • Inflammation
  • Macrophages
  • Magnetic resonance imaging
  • Monocytes
  • Nanostructures

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    Kim, J. S., An, H., Rieter, W. J., Esserman, D., Taylor-Pashow, K. M. L., Sartor, R. B., Lin, W., Lin, W., & Tarrant, T. K. (2009). Multimodal optical and Gd-based nanoparticles for imaging in inflammatory arthritis. Clinical and Experimental Rheumatology, 27(4), 580-586.