We describe multimodal nonlinear microscopy using a compact, turnkey femtosecond fiber laser at 1.5 μm. The system allows for multiplexed detection of near infrared and visible contrast agents through two-and three-photon excitation fluorescence microscopy as well as structural imaging viaauto-confocal microscopy (ACM). This platform expands the available emission spectrum for multiphoton microscopy, enables simultaneous structural and functional imaging, and offers advantages in penetration depth, contrast, and simplicity as compared to conventional MPM near 800 nm excitation.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences X
StatePublished - 2010
EventMultiphoton Microscopy in the Biomedical Sciences X - San Francisco, CA, United States
Duration: Jan 24 2010Jan 26 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceMultiphoton Microscopy in the Biomedical Sciences X
Country/TerritoryUnited States
CitySan Francisco, CA


  • Auto-confocal microscopy
  • Multiphoton microscopy
  • Near infrared fluorescence
  • Ultrafast fiber lasers


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