TY - GEN
T1 - Multimodal nonlinear microscopy at 1.5 μm
AU - Zhan, Chun
AU - Joo, Chulmin
AU - Li, Qing
AU - Berezin, Mikhail Y.
AU - Akers, Walter J.
AU - Ye, Yunpeng
AU - Achilefu, Samuel
AU - Yazdanfar, Siavash
PY - 2010
Y1 - 2010
N2 - We describe multimodal nonlinear microscopy using a compact, turnkey femtosecond fiber laser at 1.5 μm. The system allows for multiplexed detection of near infrared and visible contrast agents through two-and three-photon excitation fluorescence microscopy as well as structural imaging viaauto-confocal microscopy (ACM). This platform expands the available emission spectrum for multiphoton microscopy, enables simultaneous structural and functional imaging, and offers advantages in penetration depth, contrast, and simplicity as compared to conventional MPM near 800 nm excitation.
AB - We describe multimodal nonlinear microscopy using a compact, turnkey femtosecond fiber laser at 1.5 μm. The system allows for multiplexed detection of near infrared and visible contrast agents through two-and three-photon excitation fluorescence microscopy as well as structural imaging viaauto-confocal microscopy (ACM). This platform expands the available emission spectrum for multiphoton microscopy, enables simultaneous structural and functional imaging, and offers advantages in penetration depth, contrast, and simplicity as compared to conventional MPM near 800 nm excitation.
KW - Auto-confocal microscopy
KW - Multiphoton microscopy
KW - Near infrared fluorescence
KW - Ultrafast fiber lasers
UR - http://www.scopus.com/inward/record.url?scp=77951715246&partnerID=8YFLogxK
U2 - 10.1117/12.842573
DO - 10.1117/12.842573
M3 - Conference contribution
AN - SCOPUS:77951715246
SN - 9780819479655
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Multiphoton Microscopy in the Biomedical Sciences X
T2 - Multiphoton Microscopy in the Biomedical Sciences X
Y2 - 24 January 2010 through 26 January 2010
ER -