Multifunctional FePt nanoparticles for radiation-guided targeting and imaging of cancer

Ghazal Hariri; Matthew S. Wellons; William H. Morris; Charles M. Lukehart; Dennis E. Hallahan

doi:10.1007/s10439-010-0219-8

Multifunctional FePt nanoparticles for radiation-guided targeting and imaging of cancer

Ghazal Hariri, Matthew S. Wellons, William H. Morris, Charles M. Lukehart, Dennis E. Hallahan

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A multifunctional FePt nanoparticle was developed that targets tumor microvasculature via "radiation-guided" peptides, and is detected by both near-infrared (NIR) fluorescence imaging and analytical mass spectrometry methods. Tumor specific binding was first measured by biotinylated peptide linked to fluorophore-conjugated streptavidin. This showed tumor selective binding to tumors using the HVGGSSV peptide. FePt nanoparticles were synthesized sequentially by surface modification with poly(l)lysine, poly(ethylene) glycol conjugation, and functionalized with HVGGSSV peptide and fluorescent probe Alexa fluor 750. NIR fluorescence imaging and ICP-MS analysis showed significant HVGGSSV-FePt nanoparticle binding to irradiated tumors as compared to unirradiated tumors and controls. Results indicate that multifunctional FePt nanoparticles have potential application for radiation-guided targeting and imaging of cancer.

Original language	English
Pages (from-to)	946-952
Number of pages	7
Journal	Annals of biomedical engineering
Volume	39
Issue number	3
DOIs	https://doi.org/10.1007/s10439-010-0219-8
State	Published - Mar 2011

Keywords

Magnetic nanoparticles
Nanotechnology
Radiation
Tumor targeting

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10.1007/s10439-010-0219-8

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title = "Multifunctional FePt nanoparticles for radiation-guided targeting and imaging of cancer",

abstract = "A multifunctional FePt nanoparticle was developed that targets tumor microvasculature via {"}radiation-guided{"} peptides, and is detected by both near-infrared (NIR) fluorescence imaging and analytical mass spectrometry methods. Tumor specific binding was first measured by biotinylated peptide linked to fluorophore-conjugated streptavidin. This showed tumor selective binding to tumors using the HVGGSSV peptide. FePt nanoparticles were synthesized sequentially by surface modification with poly(l)lysine, poly(ethylene) glycol conjugation, and functionalized with HVGGSSV peptide and fluorescent probe Alexa fluor 750. NIR fluorescence imaging and ICP-MS analysis showed significant HVGGSSV-FePt nanoparticle binding to irradiated tumors as compared to unirradiated tumors and controls. Results indicate that multifunctional FePt nanoparticles have potential application for radiation-guided targeting and imaging of cancer.",

keywords = "Magnetic nanoparticles, Nanotechnology, Radiation, Tumor targeting",

author = "Ghazal Hariri and Wellons, {Matthew S.} and Morris, {William H.} and Lukehart, {Charles M.} and Hallahan, {Dennis E.}",

note = "Funding Information: This work was supported by Vanderbilt-Ingram Cancer Center, and NIH grants P50CA128323, R01-CA112385, R21-CA128456, and R01-CA125757. We thank Rossane Delapp for technical assistance.",

year = "2011",

month = mar,

doi = "10.1007/s10439-010-0219-8",

language = "English",

volume = "39",

pages = "946--952",

journal = "Annals of Biomedical Engineering",

issn = "0090-6964",

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T1 - Multifunctional FePt nanoparticles for radiation-guided targeting and imaging of cancer

AU - Hariri, Ghazal

AU - Wellons, Matthew S.

AU - Morris, William H.

AU - Lukehart, Charles M.

AU - Hallahan, Dennis E.

N1 - Funding Information: This work was supported by Vanderbilt-Ingram Cancer Center, and NIH grants P50CA128323, R01-CA112385, R21-CA128456, and R01-CA125757. We thank Rossane Delapp for technical assistance.

PY - 2011/3

Y1 - 2011/3

N2 - A multifunctional FePt nanoparticle was developed that targets tumor microvasculature via "radiation-guided" peptides, and is detected by both near-infrared (NIR) fluorescence imaging and analytical mass spectrometry methods. Tumor specific binding was first measured by biotinylated peptide linked to fluorophore-conjugated streptavidin. This showed tumor selective binding to tumors using the HVGGSSV peptide. FePt nanoparticles were synthesized sequentially by surface modification with poly(l)lysine, poly(ethylene) glycol conjugation, and functionalized with HVGGSSV peptide and fluorescent probe Alexa fluor 750. NIR fluorescence imaging and ICP-MS analysis showed significant HVGGSSV-FePt nanoparticle binding to irradiated tumors as compared to unirradiated tumors and controls. Results indicate that multifunctional FePt nanoparticles have potential application for radiation-guided targeting and imaging of cancer.

AB - A multifunctional FePt nanoparticle was developed that targets tumor microvasculature via "radiation-guided" peptides, and is detected by both near-infrared (NIR) fluorescence imaging and analytical mass spectrometry methods. Tumor specific binding was first measured by biotinylated peptide linked to fluorophore-conjugated streptavidin. This showed tumor selective binding to tumors using the HVGGSSV peptide. FePt nanoparticles were synthesized sequentially by surface modification with poly(l)lysine, poly(ethylene) glycol conjugation, and functionalized with HVGGSSV peptide and fluorescent probe Alexa fluor 750. NIR fluorescence imaging and ICP-MS analysis showed significant HVGGSSV-FePt nanoparticle binding to irradiated tumors as compared to unirradiated tumors and controls. Results indicate that multifunctional FePt nanoparticles have potential application for radiation-guided targeting and imaging of cancer.

KW - Magnetic nanoparticles

KW - Nanotechnology

KW - Radiation

KW - Tumor targeting

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DO - 10.1007/s10439-010-0219-8

M3 - Article

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AN - SCOPUS:79951549910

SN - 0090-6964

VL - 39

SP - 946

EP - 952

JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

IS - 3

ER -

Multifunctional FePt nanoparticles for radiation-guided targeting and imaging of cancer

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Keywords

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