Multi-spectral analysis of animal tissues in the second NIR window based on endogenous chromophores

Qian Cao, Steven T. Wang, Natalia G. Zhegalova, Walter J. Akers, Mikhail Y. Berezin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

To minimize the problem with scattering in deep tissues while increasing the penetration depth, we explored the feasibility of imaging in the previously unexplored extended NIR (exNIR) spectral region at 900 - 1400 nm with endogenous chromophores. This region, also known as second NIR window, is weakly dominated by absorption of water and lipids and free from other endogenous chromophores, with virtually no autofluorescence. To demonstrate the applicability of the exNIR in bioimaging, we analyzed optical properties of individual components and animal organs using InGaAs spectrophotometer and a multispectral InGaAs scanning imager featuring in transmission geometry.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
DOIs
StatePublished - May 27 2013
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI - San Francisco, CA, United States
Duration: Feb 2 2013Feb 5 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8587
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Country/TerritoryUnited States
CitySan Francisco, CA
Period02/2/1302/5/13

Keywords

  • endogenous chromophores
  • exNIR
  • lipids
  • multispectral imaging
  • water

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