Multi-photon molecular excitation in laser-scanning microscopy

Winfried Denk; David W. Piston; Watt W. Webb

doi:10.1007/978-0-387-45524-2_28

Multi-photon molecular excitation in laser-scanning microscopy

Winfried Denk, David W. Piston, Watt W. Webb

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

53 Scopus citations

Abstract

Multi-photon microscopy (MPM), which is based on molecular excitation by multi-photon absorption (MPA) and is usually combined with laser-scanning microscopy (LSM), has fulfilled its early promise (Denk et al., 1990), as evidenced by continued growth of its application to vital imaging of biological systems (for a recent collection of reprints, see Masters, 2003). Conventional fluorescence microscopy can provide submicron spatial resolution of chemical dynamics within living cells, but is frequently limited in sensitivity and spatial resolution by background due to out-offocus and scattered fluorescence.

Original language	English
Title of host publication	Handbook of Biological Confocal Microscopy
Subtitle of host publication	Third Edition
Publisher	Springer US
Pages	535-549
Number of pages	15
ISBN (Print)	038725921X, 9780387259215
DOIs	https://doi.org/10.1007/978-0-387-45524-2_28
State	Published - 2006

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10.1007/978-0-387-45524-2_28

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ER -

Multi-photon molecular excitation in laser-scanning microscopy

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