@inbook{baf9037370674c3e848dea11e7a6ef8f,
title = "Multi-photon excitation imaging of dynamic processes in living cells and tissues",
abstract = "Over the past decade, two-photon microscopy has successfully made the transition from the laser laboratory into a true biological research setting. This has been due in part to the recent development of turnkey ultrafast laser systems required for two-photon microscopy, allowing ease of use in nonspecialist laboratories. The advantages of two-photon microscopy over conventional optical sectioning techniques are for greater imaging depths and reduced overall phototoxicity, as such enabling noninvasive intra-vital imaging of cellular and subcellular processes. Greater understanding of these advantages has allowed this technique to be more effectively utilized in a biological research setting. This review will cover the recent widespread uses of two-photon microscopy and highlight the wide range of physiological studies enabled in fields such as neurosciences, developmental biology, immunology, cancer biology, and endocrinology.",
keywords = "Ablation, Fluorescence, Intra-vital imaging, Intrinsic fluorophores, Optical sectioning, Photoactivation, Second harmonic generation, Two-photon microscopy",
author = "Benninger, {R. K.P.} and M. Hao and Piston, {D. W.}",
note = "Funding Information: We are grateful to Gert-Jan Kremers for helpful comments on the manuscript. The authors acknowledge financial support from National Institute of Health grants R01-DK53434, P20-GM72048 and the Medical Free-Electron Laser Program of the United States Department of Defense. ",
year = "2008",
doi = "10.1007/112-2008-801",
language = "English",
isbn = "9783540776031",
series = "Reviews of Physiology, Biochemistry and Pharmacology",
pages = "71--92",
editor = "Simon Rallison",
booktitle = "Reviews of Physiology Biochemistry and Pharmacology",
}