TY - JOUR
T1 - Multi-centre evaluation of mass spectrometric identification of anaerobic bacteria using the VITEK® MS system
AU - Garner, O.
AU - Mochon, A.
AU - Branda, J.
AU - Burnham, C. A.
AU - Bythrow, M.
AU - Ferraro, M.
AU - Ginocchio, C.
AU - Jennemann, R.
AU - Manji, R.
AU - Procop, G. W.
AU - Richter, S.
AU - Rychert, J.
AU - Sercia, L.
AU - Westblade, L.
AU - Lewinski, M.
N1 - Funding Information:
J. Branda, J. Rychert, and M. Ferraro received research funding from bioMérieux and Becton, Dickinson. C. Ginocchio received research funding and consulting fees from bioMérieux and Becton, Dickinson. G. Procop received research funding from bioMérieux, Bruker, and Luminex. S. Richter received research funding from bioMérieux, Nanosphere, and Forest Laboratories. C. Burnham received research funding from bioMérieux, Acclerate, Cepheid, and T2 Biosystems. The other authors have no conflicts to disclose. This study was performed as part of an FDA trial of the Vitek MS v2.0 system funded by the device manufacturer bioMérieux.
PY - 2014
Y1 - 2014
N2 - Accurate and timely identification of anaerobic bacteria is critical to successful treatment. Classic phenotypic methods for identification require long turnaround times and can exhibit poor species level identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an identification method that can provide rapid identification of anaerobes. We present a multi-centre study assessing the clinical performance of the VITEK® MS in the identification of anaerobic bacteria. Five different test sites analysed a collection of 651 unique anaerobic isolates comprising 11 different genera. Multiple species were included for several of the genera. Briefly, anaerobic isolates were applied directly to a well of a target plate. Matrix solution (α-cyano-4-hydroxycinnamic acid) was added and allowed to dry. Mass spectra results were generated with the VITEK® MS, and the comparative spectral analysis and organism identification were determined using the VITEK® MS database 2.0. Results were confirmed by 16S rRNA gene sequencing. Of the 651 isolates analysed, 91.2% (594/651) exhibited the correct species identification. An additional eight isolates were correctly identified to genus level, raising the rate of identification to 92.5%. Genus-level identification consisted of Actinomyces, Bacteroides and Prevotella species. Fusobacterium nucleatum, Actinomyces neuii and Bacteroides uniformis were notable for an increased percentage of no-identification results compared with the other anaerobes tested. VITEK® MS identification of clinically relevant anaerobes is highly accurate and represents a dramatic improvement over other phenotypic methods in accuracy and turnaround time.
AB - Accurate and timely identification of anaerobic bacteria is critical to successful treatment. Classic phenotypic methods for identification require long turnaround times and can exhibit poor species level identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an identification method that can provide rapid identification of anaerobes. We present a multi-centre study assessing the clinical performance of the VITEK® MS in the identification of anaerobic bacteria. Five different test sites analysed a collection of 651 unique anaerobic isolates comprising 11 different genera. Multiple species were included for several of the genera. Briefly, anaerobic isolates were applied directly to a well of a target plate. Matrix solution (α-cyano-4-hydroxycinnamic acid) was added and allowed to dry. Mass spectra results were generated with the VITEK® MS, and the comparative spectral analysis and organism identification were determined using the VITEK® MS database 2.0. Results were confirmed by 16S rRNA gene sequencing. Of the 651 isolates analysed, 91.2% (594/651) exhibited the correct species identification. An additional eight isolates were correctly identified to genus level, raising the rate of identification to 92.5%. Genus-level identification consisted of Actinomyces, Bacteroides and Prevotella species. Fusobacterium nucleatum, Actinomyces neuii and Bacteroides uniformis were notable for an increased percentage of no-identification results compared with the other anaerobes tested. VITEK® MS identification of clinically relevant anaerobes is highly accurate and represents a dramatic improvement over other phenotypic methods in accuracy and turnaround time.
KW - Anaerobe
KW - Bacterial identification
KW - Clinical microbiology
KW - Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
KW - VITEK-MS
UR - http://www.scopus.com/inward/record.url?scp=84895922305&partnerID=8YFLogxK
U2 - 10.1111/1469-0691.12317
DO - 10.1111/1469-0691.12317
M3 - Article
C2 - 23927597
AN - SCOPUS:84895922305
VL - 20
SP - 335
EP - 339
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
SN - 1198-743X
IS - 4
ER -