MUC1 aptamer based near infrared fluorescence probes for tumor diagnosis

Juan Zhao, Yuxiang Ma, Sisi Cui, Jie Cao, Samuel Achilefu, Yueqing Gu

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

Mucin 1 (MUC1) is a cell surface mucin broadly expressed in mucosal tissues. The aberrant expression of MUC1 under-glycosylated forms has been reported in various carcinomas of the epithelium, such as breast, pancreatic and ovarian cancers. Using the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology, aptamers previously selected against MUC1 glycoprotein with high affinities and specificities. In this study, we developed two targeted near-infrared fluorescent probes for tumor in-vivo diagnostics using a MUC1 aptamer(APT) as targeted ligand and near-infrared fluorescent dye (ICG-Der-02) as labelling. MUC1 aptamer conjugated ICG-Der-02 (APT-ICG-Der-02) displayed a great selectivity to MUC1 positive cell line MCF7 and MCF7 xenograft-bearing nude mice. To improve the high targeting of the probe to the tumor cells, PEG, with high biocompatibility, non immunogenicity and long circulation, was conjugated to the probe.The new probe (APT-PEG-ICG-Der-02) showed better tumour uptake and clearance, and also displayed a great selectivity to MCF7 tumor-bearing nude mice. Data obtained demonstrate a high potential of the targeted near-infrared fluorescent probes in cancer early diagnosis.

Original languageEnglish
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
DOIs
StatePublished - 2013
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V - San Francisco, CA, United States
Duration: Feb 4 2013Feb 6 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8596
ISSN (Print)1605-7422

Conference

ConferenceReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
Country/TerritoryUnited States
CitySan Francisco, CA
Period02/4/1302/6/13

Keywords

  • Mucin 1
  • aptamer
  • imaging
  • tumor target

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