mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels

Alice Butler; Susan Tsunoda; David P. McCobb; Aguan Wei; Lawrence Salkoff

doi:10.1126/science.7687074

mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels

Alice Butler
, Susan Tsunoda
, David P. McCobb
, Aguan Wei
, Lawrence Salkoff

Research output: Contribution to journal › Article › peer-review

604 Scopus citations

Abstract

Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled "maxi" or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.

Original language	English
Pages (from-to)	221-224
Number of pages	4
Journal	Science
Volume	261
Issue number	5118
DOIs	https://doi.org/10.1126/science.7687074
State	Published - Jul 9 1993

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abstract = "Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled {"}maxi{"} or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.",

author = "Alice Butler and Susan Tsunoda and McCobb, \{David P.\} and Aguan Wei and Lawrence Salkoff",

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AU - Butler, Alice

AU - Tsunoda, Susan

AU - McCobb, David P.

AU - Wei, Aguan

AU - Salkoff, Lawrence

PY - 1993/7/9

Y1 - 1993/7/9

N2 - Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled "maxi" or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.

AB - Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled "maxi" or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.

UR - https://www.scopus.com/pages/publications/0027161159

U2 - 10.1126/science.7687074

DO - 10.1126/science.7687074

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VL - 261

SP - 221

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JO - Science

JF - Science

IS - 5118

ER -

mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels

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