mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels

Alice Butler; Susan Tsunoda; David P. McCobb; Aguan Wei; Lawrence Salkoff

doi:10.1126/science.7687074

mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels

Alice Butler, Susan Tsunoda, David P. McCobb, Aguan Wei, Lawrence Salkoff

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Abstract

Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled "maxi" or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.

Original language	English
Pages (from-to)	221-224
Number of pages	4
Journal	Science
Volume	261
Issue number	5118
DOIs	https://doi.org/10.1126/science.7687074
State	Published - Jul 9 1993

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AU - Tsunoda, Susan

AU - McCobb, David P.

AU - Wei, Aguan

AU - Salkoff, Lawrence

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AB - Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled "maxi" or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.

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mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels

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