Mouse vascular endothelium activates CD8+ T lymphocytes in a B7-dependent fashion

Daniel Kreisel, Alexander S. Krupnick, Keki R. Balsara, Markus Riha, Andrew E. Gelman, Sicco H. Popma, Wilson Y. Szeto, Laurence A. Turka, Bruce R. Rosengard

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

Despite several studies examining the contribution of allorecognition pathways to acute and chronic rejection of vascularized murine allografts, little data describing activation of alloreactive T cells by mouse vascular endothelium exist. We have used primary cultures of resting or IFN-γ-activated C57BL/6 (H-2b) vascular endothelial cells as stimulators and CD8+ T lymphocytes isolated from CBA/J (H-2k) mice as responders. Resting endothelium expressed low levels of MHC class I, which was markedly up-regulated after activation with IFN-γ. It also expressed moderate levels of CD80 at a resting state and after activation. Both resting and activated endothelium were able to induce proliferation of unprimed CD8+ T lymphocytes, with proliferation noted at earlier time points after coculture with activated endothelium. Activated endothelium was also able to induce proliferation of CD44low naive CD8+ T lymphocytes. Activated CD8+ T lymphocytes had the ability to produce IFN-γ and IL-2, acquired an effector phenotype, and showed up-regulation of the antiapoptotic protein Bcl-xL. Treatment with CTLA4-Ig led to marked reduction of T cell proliferation and a decrease in expression of Bcl-xL. Moreover, we demonstrate that nonhemopoietic cells such as vascular endothelium induce proliferation of CD8+ T lymphocytes in a B7-dependent fashion in vivo. These results suggest that vascular endothelium can act as an APC for CD8+ direct allorecognition and may, therefore, play an important role in regulating immune processes of allograft rejection.

Original languageEnglish
Pages (from-to)6154-6161
Number of pages8
JournalJournal of Immunology
Volume169
Issue number11
DOIs
StatePublished - Dec 1 2002

Fingerprint Dive into the research topics of 'Mouse vascular endothelium activates CD8<sup>+</sup> T lymphocytes in a B7-dependent fashion'. Together they form a unique fingerprint.

  • Cite this

    Kreisel, D., Krupnick, A. S., Balsara, K. R., Riha, M., Gelman, A. E., Popma, S. H., Szeto, W. Y., Turka, L. A., & Rosengard, B. R. (2002). Mouse vascular endothelium activates CD8+ T lymphocytes in a B7-dependent fashion. Journal of Immunology, 169(11), 6154-6161. https://doi.org/10.4049/jimmunol.169.11.6154