Mouse strain-specific acute respiratory effects of nicotine unrelated to nicotine metabolism

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3 Scopus citations


Plethysmograph measurement of respiratory phenotypes provides a highly sensitive means to study nicotine response in experimental model animals. We measured average respiratory frequency, tidal volume, minute volume and inspiratory time in C3H/HeJ and C57BL/6J mice subcutaneously administered 0.35 and 0.70 mg/kg nicotine. Both mouse strains showed significantly altered respiratory and locomotion phenotypes relative to saline-injected controls when administered the higher dose, but only C57BL/6J responded to the lower nicotine dose. Respiratory and locomotion phenotypes rarely differed significantly by sex. To investigate whether the strain-specific differences in nicotine sensitivity were related to differences in clearance, we followed up by measuring nicotine clearance in C3H/HeJ and C57BL/6J mice (0.35 mg/kg subcutaneous) and found sex differences in both strains, but no difference between strains.

Original languageEnglish
Pages (from-to)542-548
Number of pages7
JournalToxicology Mechanisms and Methods
Issue number7
StatePublished - Sep 2 2019


  • Respiration
  • mouse
  • nicotine
  • plethysmography
  • strain
  • ventilation


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