Mouse latent TGF-β binding protein-2: Molecular cloning and developmental expression

Jianming Fang, Xu Li, Elizabeth Smiley, Uta Francke, Robert P. Mecham, Jeffrey Bonadio

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The molecular cloning and developmental expression of mouse LTBP-2 are presented here. We established the identity of the cDNA by sequence comparison (80% identity with human LTBP-2) and by chromosome localization (mouse chromosome 12, band D, a region of conserved synteny with the human LTBP-2 gene). In contrast to LTBP-1 and LTBP-3, mouse LTBP-2 apparently is a more modular protein with proline/glycine-rich sequences always alternating with clusters of cysteine-rich structural motifs. We found for the first time that LTBP-2 gene expression in mouse embryos was restricted to cartilage perichondrium and blood vessels, a somewhat surprising result since other LTBP genes are widely expressed in rodent tissues. Therefore, mouse LTBP-2 may play a critical role in the assembly of latent TGF-β complexes in developing elastic tissues such as cartilage and blood vessel.

Original languageEnglish
Pages (from-to)219-230
Number of pages12
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1354
Issue number3
DOIs
StatePublished - Nov 20 1997

Keywords

  • Binding protein
  • Elastic connective tissue
  • Gene expression
  • Growth factor
  • Latent TGF- β binding protein
  • Transforming growth factor-β

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