TY - JOUR
T1 - Mouse latent TGF-β binding protein-2
T2 - Molecular cloning and developmental expression
AU - Fang, Jianming
AU - Li, Xu
AU - Smiley, Elizabeth
AU - Francke, Uta
AU - Mecham, Robert P.
AU - Bonadio, Jeffrey
PY - 1997/11/20
Y1 - 1997/11/20
N2 - The molecular cloning and developmental expression of mouse LTBP-2 are presented here. We established the identity of the cDNA by sequence comparison (80% identity with human LTBP-2) and by chromosome localization (mouse chromosome 12, band D, a region of conserved synteny with the human LTBP-2 gene). In contrast to LTBP-1 and LTBP-3, mouse LTBP-2 apparently is a more modular protein with proline/glycine-rich sequences always alternating with clusters of cysteine-rich structural motifs. We found for the first time that LTBP-2 gene expression in mouse embryos was restricted to cartilage perichondrium and blood vessels, a somewhat surprising result since other LTBP genes are widely expressed in rodent tissues. Therefore, mouse LTBP-2 may play a critical role in the assembly of latent TGF-β complexes in developing elastic tissues such as cartilage and blood vessel.
AB - The molecular cloning and developmental expression of mouse LTBP-2 are presented here. We established the identity of the cDNA by sequence comparison (80% identity with human LTBP-2) and by chromosome localization (mouse chromosome 12, band D, a region of conserved synteny with the human LTBP-2 gene). In contrast to LTBP-1 and LTBP-3, mouse LTBP-2 apparently is a more modular protein with proline/glycine-rich sequences always alternating with clusters of cysteine-rich structural motifs. We found for the first time that LTBP-2 gene expression in mouse embryos was restricted to cartilage perichondrium and blood vessels, a somewhat surprising result since other LTBP genes are widely expressed in rodent tissues. Therefore, mouse LTBP-2 may play a critical role in the assembly of latent TGF-β complexes in developing elastic tissues such as cartilage and blood vessel.
KW - Binding protein
KW - Elastic connective tissue
KW - Gene expression
KW - Growth factor
KW - Latent TGF- β binding protein
KW - Transforming growth factor-β
UR - http://www.scopus.com/inward/record.url?scp=0031459117&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(97)00104-8
DO - 10.1016/S0167-4781(97)00104-8
M3 - Article
C2 - 9427531
AN - SCOPUS:0031459117
VL - 1354
SP - 219
EP - 230
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 3
ER -