TY - JOUR
T1 - Mouse latent TGF-β binding protein-2
T2 - Molecular cloning and developmental expression
AU - Fang, Jianming
AU - Li, Xu
AU - Smiley, Elizabeth
AU - Francke, Uta
AU - Mecham, Robert P.
AU - Bonadio, Jeffrey
N1 - Funding Information:
This work was supported in part by a Sponsored Research Agreement from Matrigen Inc., the National Institutes of Health, and the Howard Hughes Medical Institute. The authors wish to thank Dr. Wushan Yin for his guidance during the early stages of this project.
PY - 1997/11/20
Y1 - 1997/11/20
N2 - The molecular cloning and developmental expression of mouse LTBP-2 are presented here. We established the identity of the cDNA by sequence comparison (80% identity with human LTBP-2) and by chromosome localization (mouse chromosome 12, band D, a region of conserved synteny with the human LTBP-2 gene). In contrast to LTBP-1 and LTBP-3, mouse LTBP-2 apparently is a more modular protein with proline/glycine-rich sequences always alternating with clusters of cysteine-rich structural motifs. We found for the first time that LTBP-2 gene expression in mouse embryos was restricted to cartilage perichondrium and blood vessels, a somewhat surprising result since other LTBP genes are widely expressed in rodent tissues. Therefore, mouse LTBP-2 may play a critical role in the assembly of latent TGF-β complexes in developing elastic tissues such as cartilage and blood vessel.
AB - The molecular cloning and developmental expression of mouse LTBP-2 are presented here. We established the identity of the cDNA by sequence comparison (80% identity with human LTBP-2) and by chromosome localization (mouse chromosome 12, band D, a region of conserved synteny with the human LTBP-2 gene). In contrast to LTBP-1 and LTBP-3, mouse LTBP-2 apparently is a more modular protein with proline/glycine-rich sequences always alternating with clusters of cysteine-rich structural motifs. We found for the first time that LTBP-2 gene expression in mouse embryos was restricted to cartilage perichondrium and blood vessels, a somewhat surprising result since other LTBP genes are widely expressed in rodent tissues. Therefore, mouse LTBP-2 may play a critical role in the assembly of latent TGF-β complexes in developing elastic tissues such as cartilage and blood vessel.
KW - Binding protein
KW - Elastic connective tissue
KW - Gene expression
KW - Growth factor
KW - Latent TGF- β binding protein
KW - Transforming growth factor-β
UR - http://www.scopus.com/inward/record.url?scp=0031459117&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(97)00104-8
DO - 10.1016/S0167-4781(97)00104-8
M3 - Article
C2 - 9427531
AN - SCOPUS:0031459117
SN - 0167-4781
VL - 1354
SP - 219
EP - 230
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 3
ER -