Morphological, phylogenetic and biological characteristics of Ectropis obliqua single-nucleocapsid nucleopolyhedrovirus

Xiu Cui Ma, Hai Jun Xu, Mei Jun Tang, Qiang Xiao, Jian Hong, Chuan Xi Zhang

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The tea looper caterpillar, Ectropis obliqua, is one of the major pests of tea bushes. E. obliqua single-nucleocapsid nucleopolyhedrovirus (EcobSNPV) has been used as a commercial pesticide for biocontrol of this insect. However only limited genetic analysis for this important virus has been done up to now. EcobSNPV was characterized in this study. Electron microscopy analysis of the occlusion body showed polyhedra of 0.7 to 1.7 μm in diameter containing a single nucleocapsid per envelope of the virion. A 15.5 kb genomic fragment containing EcoRI-L, EcoRI-N and HindIII-F fragments, was sequenced. Analysis of the sequence revealed that the fragment contained eleven potential open reading frames (ORFs): lef-1, egt, 38.7k, rr1, polyhedrin, orf1629, pk-1, hoar and homologues to Spodoptera exigua multicapsid NPV (SeMNPV) ORFs 15, 28, and 29. Gene arrangement and phylogeny analysis suggest that EcobSNPV is closely related to the previously described Group II NPV. Bioassays on lethal concentration (LC50 and LC90) and lethal time (LT50 and LT90) were conducted to test the susceptibility of E. obliqua larvae to the virus.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalJournal of Microbiology
Volume44
Issue number1
StatePublished - 2006

Keywords

  • Bioassay
  • Ectropis obliqua
  • Phylogeny
  • Polyhedrin
  • Single-nucleocapsid nucleopolyhedrovirus

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