Morphologic Design of Silver-Bearing Sugar-Based Polymer Nanoparticles for Uroepithelial Cell Binding and Antimicrobial Delivery

Yue Song, Mahmoud Elsabahy, Christina A. Collins, Sarosh Khan, Richen Li, Teri N. Hreha, Yidan Shen, Yen Nan Lin, Rachel A. Letteri, Lu Su, Mei Dong, Fuwu Zhang, David A. Hunstad, Karen L. Wooley

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Platelet-like and cylindrical nanostructures from sugar-based polymers are designed to mimic the aspect ratio of bacteria and achieve uroepithelial cell binding and internalization, thereby improving their potential for local treatment of recurrent urinary tract infections. Polymer nanostructures, derived from amphiphilic block polymers composed of zwitterionic poly(d-glucose carbonate) and semicrystalline poly(l-lactide) segments, were constructed with morphologies that could be tuned to enhance uroepithelial cell binding. These nanoparticles exhibited negligible cytotoxicity, immunotoxicity, and cytokine adsorption, while also offering substantial silver cation loading capacity, extended release, and in vitro antimicrobial activity (as effective as free silver cations) against uropathogenic Escherichia coli. In comparison to spherical analogues, cylindrical and platelet-like nanostructures engaged in significantly higher association with uroepithelial cells, as measured by flow cytometry; despite their larger size, platelet-like nanostructures maintained the capacity for cell internalization. This work establishes initial evidence of degradable platelet-shaped nanostructures as versatile therapeutic carriers for treatment of epithelial infections.

Original languageEnglish
Pages (from-to)4990-4998
Number of pages9
JournalNano Letters
Volume21
Issue number12
DOIs
StatePublished - Jun 23 2021

Keywords

  • antimicrobial
  • cell-binding and internalization
  • platelet-shaped nanostructures
  • Silver-bearing polymer nanostructures
  • urinary tract infections

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