TY - JOUR
T1 - Morphogenic potentials of D2, D3, and D4 dopamine receptors revealed in transfected neuronal cell lines
AU - Swarzenski, Barbara C.
AU - Tang, Lei
AU - Oh, Young J.
AU - O'Malley, Karen L.
AU - Todd, Richard D.
PY - 1994/1/18
Y1 - 1994/1/18
N2 - Molecular cloning studies have defined a family of dopamine D2-like receptors (D2, D3, and D4), which are the products of separate genes. Our previous work has shown that stimulation of dopamine D2-like receptors in cultures of fetal cortical neurons increases the extension and branching of neurites. To determine which D2-like receptors possess morphogenic potentials, a clonal mesencephalic cell line (MN9D) was transfected with D2, D3, or D4 receptor subtypes and treated with quinpirole, an agonist of D2- like receptors, and changes in morphological characteristics were quantitated. Stimulation of D2 receptors increased the number and branching of neurites with little effect on neurite extension; stimulation of D3 and D4 receptors increased the branching and extension of neurites. Similar results were found for primary mesencephalic cultures stimulated with quinpirole. These results suggest that the known D2-like receptors have specific developmental roles in regulating neuronal morphogenesis of dopaminergic pathways.
AB - Molecular cloning studies have defined a family of dopamine D2-like receptors (D2, D3, and D4), which are the products of separate genes. Our previous work has shown that stimulation of dopamine D2-like receptors in cultures of fetal cortical neurons increases the extension and branching of neurites. To determine which D2-like receptors possess morphogenic potentials, a clonal mesencephalic cell line (MN9D) was transfected with D2, D3, or D4 receptor subtypes and treated with quinpirole, an agonist of D2- like receptors, and changes in morphological characteristics were quantitated. Stimulation of D2 receptors increased the number and branching of neurites with little effect on neurite extension; stimulation of D3 and D4 receptors increased the branching and extension of neurites. Similar results were found for primary mesencephalic cultures stimulated with quinpirole. These results suggest that the known D2-like receptors have specific developmental roles in regulating neuronal morphogenesis of dopaminergic pathways.
KW - mesencephalic cells
KW - neuronal development
UR - http://www.scopus.com/inward/record.url?scp=0028179609&partnerID=8YFLogxK
U2 - 10.1073/pnas.91.2.649
DO - 10.1073/pnas.91.2.649
M3 - Article
C2 - 7904756
AN - SCOPUS:0028179609
SN - 0027-8424
VL - 91
SP - 649
EP - 653
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 2
ER -