TY - JOUR
T1 - Monohydroxyeicosatetraenoic acids (HETEs) induce degranulation of human neutrophils
AU - Stenson, W. F.
AU - Parker, C. W.
PY - 1980/1/1
Y1 - 1980/1/1
N2 - 5-S-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE), a lipoxygenase product in neurophils, and 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE), a lipoxygenase product in platelets, were prepared biosynthetically and incubated with purified human peripheral neutrophils. Both 5-HETE and 12-HETE at 5- to 10-μM concentrations induced degranulation of specific granules. Both 5-HETE and 12-HETE are chemotactic for neutrophils at these concentrations. Arachidonic acid, the metabolic precursor of 5-HETE and 12-HETE, did not induce degranulation. The calcium ionophore A23187 induces neutrophils degranulation and also activates arachidonic acid metabolism in neutrophils resulting in 5-HETE production. Both ionophore-induced 5-HETE production and ionophore-induced degranulation can be inhibited, with very similar inhibition curves, by 5,8,11,14-eicosatetraynoic acid, an inhibitor of arachidonate metabolism. We propose that the mechanism for 5-HETE- and 12-HETE-induced degranulation is an alteration in the fatty acid composition of membrane phospholipids.
AB - 5-S-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE), a lipoxygenase product in neurophils, and 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE), a lipoxygenase product in platelets, were prepared biosynthetically and incubated with purified human peripheral neutrophils. Both 5-HETE and 12-HETE at 5- to 10-μM concentrations induced degranulation of specific granules. Both 5-HETE and 12-HETE are chemotactic for neutrophils at these concentrations. Arachidonic acid, the metabolic precursor of 5-HETE and 12-HETE, did not induce degranulation. The calcium ionophore A23187 induces neutrophils degranulation and also activates arachidonic acid metabolism in neutrophils resulting in 5-HETE production. Both ionophore-induced 5-HETE production and ionophore-induced degranulation can be inhibited, with very similar inhibition curves, by 5,8,11,14-eicosatetraynoic acid, an inhibitor of arachidonate metabolism. We propose that the mechanism for 5-HETE- and 12-HETE-induced degranulation is an alteration in the fatty acid composition of membrane phospholipids.
UR - http://www.scopus.com/inward/record.url?scp=0018829488&partnerID=8YFLogxK
M3 - Article
C2 - 6767782
AN - SCOPUS:0018829488
VL - 124
SP - 2100
EP - 2104
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 5
ER -