TY - JOUR
T1 - Monoclonal antibody with high affinity for 1,25-dihydroxycholecalciferol
AU - Perry, Horace M.
AU - Chappel, Jean C.
AU - Clevinger, Brian L.
AU - Haddad, John G.
AU - Teitelbaum, Steven L.
PY - 1983/4/29
Y1 - 1983/4/29
N2 - We have developed a monoclonal antibody capable of detecting 1 pg/ml of 1,25-dihydroxycholecalciferol. At a dilution of 1:80,000 of ascitic fluid this antibody has an apparent KD of 3.3 × 10-11ML-1. The immunogen used was a vitamin D analogue, calcitroic acid [1α, 3 β-dihydroxy-9, 10 seco-24-nor 5,7,10 (19) cholatriene-23-oic acid], conjugated to bovine serum albumin. Although this antibody is extremely sensitive, it also recognizes other important vitamin D3 metabolites.
AB - We have developed a monoclonal antibody capable of detecting 1 pg/ml of 1,25-dihydroxycholecalciferol. At a dilution of 1:80,000 of ascitic fluid this antibody has an apparent KD of 3.3 × 10-11ML-1. The immunogen used was a vitamin D analogue, calcitroic acid [1α, 3 β-dihydroxy-9, 10 seco-24-nor 5,7,10 (19) cholatriene-23-oic acid], conjugated to bovine serum albumin. Although this antibody is extremely sensitive, it also recognizes other important vitamin D3 metabolites.
UR - http://www.scopus.com/inward/record.url?scp=0020538196&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(83)91482-1
DO - 10.1016/0006-291X(83)91482-1
M3 - Article
C2 - 6687801
AN - SCOPUS:0020538196
VL - 112
SP - 431
EP - 436
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -