Monoclonal antibody with high affinity for 1,25-dihydroxycholecalciferol

Horace M. Perry, Jean C. Chappel, Brian L. Clevinger, John G. Haddad, Steven L. Teitelbaum

Research output: Contribution to journalArticlepeer-review

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Abstract

We have developed a monoclonal antibody capable of detecting 1 pg/ml of 1,25-dihydroxycholecalciferol. At a dilution of 1:80,000 of ascitic fluid this antibody has an apparent KD of 3.3 × 10-11ML-1. The immunogen used was a vitamin D analogue, calcitroic acid [1α, 3 β-dihydroxy-9, 10 seco-24-nor 5,7,10 (19) cholatriene-23-oic acid], conjugated to bovine serum albumin. Although this antibody is extremely sensitive, it also recognizes other important vitamin D3 metabolites.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume112
Issue number2
DOIs
StatePublished - Apr 29 1983

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