Monitoring urea transport in rat kidney in vivo using hyperpolarized 13C magnetic resonance imaging

Cornelius von Morze, Robert A. Bok, Jeff M. Sands, John Kurhanewicz, Daniel B. Vigneron

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Urea functions as a key os-molyte in the urinary concentrating mechanism of the inner medulla. The urea transporter UT-A1 is upregulated by antidiuretic hormone, facilitating faster equilibration of urea between the lumen and interstitium of the inner medullary collecting duct, resulting in the formation of more highly concentrated urine. New methods in dynamic nuclear polarization, providing ~50,000-fold enhancement of nuclear magnetic resonance signals in the liquid state, offer a novel means to monitor this process in vivo using magnetic resonance imaging. In this study, we detected significant signal differences in the rat kidney between acute diuretic and antidiuretic states, using dynamic 13C magnetic resonance imaging following a bolus infusion of hyperpolarized [13C]urea. More rapid medullary enhancement was observed under antidiuresis, consistent with known upregulation of UT-A1.

Original languageEnglish
Pages (from-to)F1658-F1662
JournalAmerican Journal of Physiology - Renal Physiology
Volume302
Issue number12
DOIs
StatePublished - Jun 15 2012
Externally publishedYes

Keywords

  • Diuresis
  • Dynamic nuclear polarization
  • UT-A1
  • Urinary concentrating mechanism

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