Monitoring notch activation in cultured mammalian cells: Transcriptional reporter assays

Ma Xenia G. Ilagan, Raphael Kopan

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

Upon ligand binding, Notch activation and cleavage culminates in the expression of its target genes. Hence, the use of Notch-responsive promoters to drive reporter gene expression provides a flexible and robust approach for monitoring signaling activity. In this chapter, we present an overview of Notch transcriptional reporter assays and discuss different methods for ligand-independent and ligand-dependent activation of Notch in mammalian cells.

Original languageEnglish
Title of host publicationNotch Signaling
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages143-154
Number of pages12
ISBN (Print)9781493911387
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1187
ISSN (Print)1064-3745

Keywords

  • Calcium chelation
  • Immobilized ligand
  • Ligand co-culture
  • Luciferase
  • Notch
  • Reporter assay
  • Transcription

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