@inbook{96c55b19ee2b48448107bb547141c844,
title = "Monitoring notch activation in cultured mammalian cells: Transcriptional reporter assays",
abstract = "Upon ligand binding, Notch activation and cleavage culminates in the expression of its target genes. Hence, the use of Notch-responsive promoters to drive reporter gene expression provides a flexible and robust approach for monitoring signaling activity. In this chapter, we present an overview of Notch transcriptional reporter assays and discuss different methods for ligand-independent and ligand-dependent activation of Notch in mammalian cells.",
keywords = "Calcium chelation, Immobilized ligand, Ligand co-culture, Luciferase, Notch, Reporter assay, Transcription",
author = "Ilagan, {Ma Xenia G.} and Raphael Kopan",
year = "2014",
doi = "10.1007/978-1-4939-1139-4_11",
language = "English",
isbn = "9781493911387",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "143--154",
booktitle = "Notch Signaling",
}