TY - JOUR
T1 - Monitoring insulin-stimulated production of signaling lipids at the plasma membrane.
AU - Osisami, Mary
AU - Huang, Huiyan
AU - Frohman, Michael A.
PY - 2009
Y1 - 2009
N2 - Lipid second messengers play important roles in many cell signaling cascades. Lipid signaling molecules allow for high specificity, rapid transduction, and rapid reversibility of localized stimulation events. Fluorescent sensors capable of detecting individual signaling lipids enable their production and degradation to be followed, revealing the nature and dynamics of signaling pathways. The following sections outline a method for using lipid sensors to monitor the production of signaling lipids on the plasma membrane of C2C12 myotubes in response to insulin signaling.
AB - Lipid second messengers play important roles in many cell signaling cascades. Lipid signaling molecules allow for high specificity, rapid transduction, and rapid reversibility of localized stimulation events. Fluorescent sensors capable of detecting individual signaling lipids enable their production and degradation to be followed, revealing the nature and dynamics of signaling pathways. The following sections outline a method for using lipid sensors to monitor the production of signaling lipids on the plasma membrane of C2C12 myotubes in response to insulin signaling.
UR - https://www.scopus.com/pages/publications/74949085267
U2 - 10.1007/978-1-60327-378-7_4
DO - 10.1007/978-1-60327-378-7_4
M3 - Article
C2 - 19763497
AN - SCOPUS:74949085267
SN - 1064-3745
VL - 590
SP - 61
EP - 70
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -