Molecular thermometers for potential applications in thermal ablation procedures

Natalia G. Zhegalova, Alex Aydt, Steven T. Wang, Mikhail Y. Berezin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Thermal ablation is a promising minimally invasive method for treating tumors without surgical intervention. Thermal ablation uses thermal sources such as lasers, radiowaves or focused ultrasound to increase the temperature of the tumor to levels lethal to cancer cells. This treatment based on heat therapy may be problematic as the temperature of the operation site is unknown. To address this problem, we developed optical molecular thermometers that can potentially measure the temperature on a molecular scale and be compatible with in vivo measurements. The thermometers are centered on a combination of two fluorophores emitting in two distinct spectral ranges and having different temperature-dependent emission properties. In this design, a fluorophore with relatively insensitive temperature-dependent fluorescence serves as a reference while another sensitive fluorophore serves as a sensor. We have demonstrated the feasibility of this approach using a coumarin-rhodamine conjugate. The sensitivity of the construct to the clinically relevant ablation temperatures (20-85°C) was demonstrated in vitro.

Original languageEnglish
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
DOIs
StatePublished - May 30 2013
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V - San Francisco, CA, United States
Duration: Feb 4 2013Feb 6 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8596
ISSN (Print)1605-7422

Conference

ConferenceReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
CountryUnited States
CitySan Francisco, CA
Period02/4/1302/6/13

Keywords

  • coumarin
  • fluorescence
  • molecular thermometers
  • rhodamine
  • thermal ablation

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  • Cite this

    Zhegalova, N. G., Aydt, A., Wang, S. T., & Berezin, M. Y. (2013). Molecular thermometers for potential applications in thermal ablation procedures. In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V [85960I] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8596). https://doi.org/10.1117/12.2008784