Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches

Marios Giannakis, Thaddeus Stappenbeck, Jason C. Mills, Douglas G. Leip, Michael Lovett, Sandra W. Clifton, Joseph E. Ippolito, Jarret I. Glasscock, Manimozhiyan Arumugam, Michael R. Brent, Jeffrey I. Gordon

Research output: Contribution to journalArticlepeer-review

153 Scopus citations


We have sequenced 36,641 expressed sequence tags from laser capture microdissected adult mouse gastric and small intestinal epithelial progenitors, obtaining 4031 and 3324 unique transcripts, respectively. Using Gene Ontology (GO) terms, each data set was compared with cDNA libraries from intact adult stomach and small intestine. Genes in GO categories enriched in progenitors were filtered against genes in GO categories represented in hematopoietic, neural, and embryonic stem cell transcriptomes and mapped onto transcription factor networks, plus canonical signal transduction and metabolic pathways. Wnt/β-catenin, phosphoinositide-3/Akt kinase, insulin-like growth factor-1, vascular endothelial growth factor, integrin, and γ-aminobutyric acid receptor signaling cascades, plus glycerolipid, fatty acid, and amino acid metabolic pathways are among those prominently represented in adult gut progenitors. The results reveal shared as well as distinctive features of adult gut stem cells when compared with other stem cell populations.

Original languageEnglish
Pages (from-to)11292-11300
Number of pages9
JournalJournal of Biological Chemistry
Issue number16
StatePublished - Apr 21 2006


Dive into the research topics of 'Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches'. Together they form a unique fingerprint.

Cite this