Molecular imaging using a site-targeted ultrasound contrast agent

Jon N. Marsh; Christopher S. Hall; Dana R. Abendschein; David E. Scherrer; Michael J. Scott; Ralph J. Fuhrhop; Patrick J. Gaffney; Samuel A. Wickline; Gregory M. Lanza

Molecular imaging using a site-targeted ultrasound contrast agent

Jon N. Marsh, Christopher S. Hall, Dana R. Abendschein, David E. Scherrer, Michael J. Scott, Ralph J. Fuhrhop, Patrick J. Gaffney, Samuel A. Wickline, Gregory M. Lanza

Research output: Contribution to journal › Conference article › peer-review

2 Scopus citations

Abstract

We have developed a nongaseous, ligand-targeted perfluorocarbon nanoparticle emulsion which can acoustically enhance the presence of molecular epitopes on tissue surfaces. In a two-part study, we first demonstrate how perfluorocarbons with varying phase velocities impact the acoustic reflectivity of plasma clots targeted with an anti-fibrin perfluorocarbon nanoparticle in vitro. The targeted perfluorocarbon contrast agents increased backscattered power from 18.2 to 23.4 dB with the greatest enhancement noted for formulations with low acoustic impedance. Porcine carotid arteries were injured by percutaneous balloon angioplasty and treated in situ with a tissue factor-targeted or control nanoparticulate immunoemulsion. Intravascular ultrasound images (30 MHz) of the arteries before and after application of contrast agent demonstrated the acoustic localization and enhancement of stretch-induced tissue factor expression within the carotid media, which was not apparent in the control vessels.

Original language	English
Pages (from-to)	1713-1716
Number of pages	4
Journal	Proceedings of the IEEE Ultrasonics Symposium
Volume	2
State	Published - 1999
Event	1999 IEEE Ultrasonics Symposium - Caesars Tahoe, NV, USA Duration: Oct 17 1999 → Oct 20 1999

Cite this

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title = "Molecular imaging using a site-targeted ultrasound contrast agent",

abstract = "We have developed a nongaseous, ligand-targeted perfluorocarbon nanoparticle emulsion which can acoustically enhance the presence of molecular epitopes on tissue surfaces. In a two-part study, we first demonstrate how perfluorocarbons with varying phase velocities impact the acoustic reflectivity of plasma clots targeted with an anti-fibrin perfluorocarbon nanoparticle in vitro. The targeted perfluorocarbon contrast agents increased backscattered power from 18.2 to 23.4 dB with the greatest enhancement noted for formulations with low acoustic impedance. Porcine carotid arteries were injured by percutaneous balloon angioplasty and treated in situ with a tissue factor-targeted or control nanoparticulate immunoemulsion. Intravascular ultrasound images (30 MHz) of the arteries before and after application of contrast agent demonstrated the acoustic localization and enhancement of stretch-induced tissue factor expression within the carotid media, which was not apparent in the control vessels.",

author = "Marsh, {Jon N.} and Hall, {Christopher S.} and Abendschein, {Dana R.} and Scherrer, {David E.} and Scott, {Michael J.} and Fuhrhop, {Ralph J.} and Gaffney, {Patrick J.} and Wickline, {Samuel A.} and Lanza, {Gregory M.}",

year = "1999",

language = "English",

volume = "2",

pages = "1713--1716",

journal = "Proceedings of the IEEE Ultrasonics Symposium",

issn = "1051-0117",

}

Molecular imaging using a site-targeted ultrasound contrast agent. / Marsh, Jon N.; Hall, Christopher S.; Abendschein, Dana R.; Scherrer, David E.; Scott, Michael J.; Fuhrhop, Ralph J.; Gaffney, Patrick J.; Wickline, Samuel A.; Lanza, Gregory M.

In: Proceedings of the IEEE Ultrasonics Symposium, Vol. 2, 1999, p. 1713-1716.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Molecular imaging using a site-targeted ultrasound contrast agent

AU - Marsh, Jon N.

AU - Hall, Christopher S.

AU - Abendschein, Dana R.

AU - Scherrer, David E.

AU - Scott, Michael J.

AU - Fuhrhop, Ralph J.

AU - Gaffney, Patrick J.

AU - Wickline, Samuel A.

AU - Lanza, Gregory M.

PY - 1999

Y1 - 1999

N2 - We have developed a nongaseous, ligand-targeted perfluorocarbon nanoparticle emulsion which can acoustically enhance the presence of molecular epitopes on tissue surfaces. In a two-part study, we first demonstrate how perfluorocarbons with varying phase velocities impact the acoustic reflectivity of plasma clots targeted with an anti-fibrin perfluorocarbon nanoparticle in vitro. The targeted perfluorocarbon contrast agents increased backscattered power from 18.2 to 23.4 dB with the greatest enhancement noted for formulations with low acoustic impedance. Porcine carotid arteries were injured by percutaneous balloon angioplasty and treated in situ with a tissue factor-targeted or control nanoparticulate immunoemulsion. Intravascular ultrasound images (30 MHz) of the arteries before and after application of contrast agent demonstrated the acoustic localization and enhancement of stretch-induced tissue factor expression within the carotid media, which was not apparent in the control vessels.

AB - We have developed a nongaseous, ligand-targeted perfluorocarbon nanoparticle emulsion which can acoustically enhance the presence of molecular epitopes on tissue surfaces. In a two-part study, we first demonstrate how perfluorocarbons with varying phase velocities impact the acoustic reflectivity of plasma clots targeted with an anti-fibrin perfluorocarbon nanoparticle in vitro. The targeted perfluorocarbon contrast agents increased backscattered power from 18.2 to 23.4 dB with the greatest enhancement noted for formulations with low acoustic impedance. Porcine carotid arteries were injured by percutaneous balloon angioplasty and treated in situ with a tissue factor-targeted or control nanoparticulate immunoemulsion. Intravascular ultrasound images (30 MHz) of the arteries before and after application of contrast agent demonstrated the acoustic localization and enhancement of stretch-induced tissue factor expression within the carotid media, which was not apparent in the control vessels.

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M3 - Conference article

AN - SCOPUS:0033294631

VL - 2

SP - 1713

EP - 1716

JO - Proceedings of the IEEE Ultrasonics Symposium

JF - Proceedings of the IEEE Ultrasonics Symposium

SN - 1051-0117

Y2 - 17 October 1999 through 20 October 1999

ER -

Molecular imaging using a site-targeted ultrasound contrast agent

Abstract

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