Molecular epidemiology of Escherichia coli O157:H7 strains by bacteriophage λ restriction fragment length polymorphism analysis: Application to a multistate foodborne outbreak and a day-care center cluster

M. Samadpour, L. M. Grimm, B. Desai, D. Alfi, J. E. Ongerth, P. I. Tarr

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Genomic DNAs prepared from 168 isolates of Escherichia coli O157:H7 were analyzed for restriction fragment length polymorphisms on Southern blots probed with bacteriophage λ DNA. The isolates analyzed included strains from a recent large multistate outbreak of E. coli O157:H7 infection associated with consumption of poorly cooked beef in restaurants, a day-care center cluster, and temporally and geographically unrelated isolates. E. coli O157:H7 isolates recovered from the incriminated meat and from 61 (96.8%) of 63 patients from Washington and Nevada possessed identical λ restriction fragment length patterns. The λ restriction fragment length polymorphisms observed in 11 (91.7%) of 12 day-care center patients were identical, but they differed from that of the strain associated with the multistate outbreak. E. coli O157:H7 from 42 patients temporally or geographically unrelated to either cluster of infection possessed unique and different λ restriction fragment length patterns, except for paired isolates from three separate clusters of infection. These data demonstrate that the hybridization of DNA digests of E. coli O157:H7 with radiolabelled bacteriophage λ DNA can be a useful, stable, and discriminatory epidemiologic tool for analyzing the linkage between strains of E. coli O157:H7.

Original languageEnglish
Pages (from-to)3179-3183
Number of pages5
JournalJournal of clinical microbiology
Volume31
Issue number12
StatePublished - Jan 1 1993
Externally publishedYes

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