TY - JOUR
T1 - Molecular cloning, structure and expression of the yeast proliferating cell nuclear antigen gene
AU - Bauer, Glenn A.
AU - Burgers, M. J.
N1 - Funding Information:
We thank Kim Percival for excellent technical assistance, John Majors for helpful suggestions, and Jim Dutchik and Maynard Olson for physical mapping of the POL30 gene. Protein sequence analysis and oligonucleotide synthesis was carried out in the Protein Chemistry Laboratory of Washington University School of Medicine. Supported by a grant from the National Institutes of Health. P.M.J.B. is an established investigator of the American Heart Association.
PY - 1990/1/25
Y1 - 1990/1/25
N2 - The budding yeast Saccharomyces cerevisiae is proving to be an useful and accurate model for eukaryotic DNA replication. It contains both DNA polymerase α (I) and δ (III). Recently, proliferating cell nuclear antigen (PCNA), which in mammalian cells is an auxiliary subunit of DNA polymerase 6 and is essential for in vitro leading strand SV40 DNA replication, was purified from yeast. We have now cloned the gene for yeast PCNA (POL30). The gene codes for an essential protein of 29 kDa, which shows 35% homology with human PCNA. Cell cycle expression studies, using synchronized cells, show that expression of both the PCNA (POL30) and the DNA polymerase δ (POL3, or CDC2) genes of yeast are regulated in an identical fashion to that of the DNA polymerase a (POL1) gene. Thus, steady state mRNA levels increase 10-100-fold in late G1 phase, peak in early S-phase, and decrease to low levels in late Sphase. In addition, in meiosis mRNA levels increase prior to initiation of premeiotic DNA synthesis.
AB - The budding yeast Saccharomyces cerevisiae is proving to be an useful and accurate model for eukaryotic DNA replication. It contains both DNA polymerase α (I) and δ (III). Recently, proliferating cell nuclear antigen (PCNA), which in mammalian cells is an auxiliary subunit of DNA polymerase 6 and is essential for in vitro leading strand SV40 DNA replication, was purified from yeast. We have now cloned the gene for yeast PCNA (POL30). The gene codes for an essential protein of 29 kDa, which shows 35% homology with human PCNA. Cell cycle expression studies, using synchronized cells, show that expression of both the PCNA (POL30) and the DNA polymerase δ (POL3, or CDC2) genes of yeast are regulated in an identical fashion to that of the DNA polymerase a (POL1) gene. Thus, steady state mRNA levels increase 10-100-fold in late G1 phase, peak in early S-phase, and decrease to low levels in late Sphase. In addition, in meiosis mRNA levels increase prior to initiation of premeiotic DNA synthesis.
UR - https://www.scopus.com/pages/publications/0025073229
U2 - 10.1093/nar/18.2.261
DO - 10.1093/nar/18.2.261
M3 - Article
C2 - 1970160
AN - SCOPUS:0025073229
SN - 0305-1048
VL - 18
SP - 261
EP - 265
JO - Nucleic acids research
JF - Nucleic acids research
IS - 2
ER -