Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1

P. M. Handley; M. Mueckler; N. R. Siegel; A. Ciechanover; A. L. Schwartz

doi:10.1073/pnas.88.1.258

Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1

P. M. Handley, M. Mueckler, N. R. Siegel, A. Ciechanover, A. L. Schwartz

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Abstract

The ubiquitin-activating enzyme E1 catalyzes the first step in ubiquitin conjugation. We have cloned and sequenced the cDNA for human E1. This clone predicts a protein of 110,450 Da. Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein E2, suggesting a potential role for this region in enzymatic function of this protein. In addition, Cys-454 lies within a region of identity to the thiol ester consensus sequence of several proteins involved in thioester formation. Tissue distribution reveals a single 3.5-kilobase E1 message ubiquitous among tissues and cell lines.

Original language	English
Pages (from-to)	258-262
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	88
Issue number	1
DOIs	https://doi.org/10.1073/pnas.88.1.258
State	Published - 1991

Keywords

protein degradation

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abstract = "The ubiquitin-activating enzyme E1 catalyzes the first step in ubiquitin conjugation. We have cloned and sequenced the cDNA for human E1. This clone predicts a protein of 110,450 Da. Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein E2, suggesting a potential role for this region in enzymatic function of this protein. In addition, Cys-454 lies within a region of identity to the thiol ester consensus sequence of several proteins involved in thioester formation. Tissue distribution reveals a single 3.5-kilobase E1 message ubiquitous among tissues and cell lines.",

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AU - Ciechanover, A.

AU - Schwartz, A. L.

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AB - The ubiquitin-activating enzyme E1 catalyzes the first step in ubiquitin conjugation. We have cloned and sequenced the cDNA for human E1. This clone predicts a protein of 110,450 Da. Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein E2, suggesting a potential role for this region in enzymatic function of this protein. In addition, Cys-454 lies within a region of identity to the thiol ester consensus sequence of several proteins involved in thioester formation. Tissue distribution reveals a single 3.5-kilobase E1 message ubiquitous among tissues and cell lines.

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JF - Proceedings of the National Academy of Sciences of the United States of America

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Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1

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Keywords

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