Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2

Vivian Zhang, Mary O'Sullivan, Hameda Hussain, William T. Roswit, Michael J. Holtzman

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Structural characterization for ovine prostaglandin H synthase-1 (PGHS-1) is extensive, but the corre spending structure for the homologous ovine PGHS-2 isoform is undefined. Accordingly, we isolated a full-length (3.4 kb) ovine PGHS-2 cDNA from a primary-culture cell model (ovine tracheal epithelial cells) originally described as containing both PGHS isoforms. Analysis of ovine PGHS-2 cDNA sequence indicated conservation of critical amino acid residues, but differences in other hydrophilic regions allowed for the development of an anti-peptide antibody highly selective for PGHS-2. Enzymatic activities of the recombinant ovine PGHS isozymes indicated significant differences in response to aspirin-acetylation consistent with the characteristics of endogenous cellular PGHS activities under basal and serum-induced conditions. The results fully account for previous evidence of two distinct PGHS activities in cultured airway epithelial cells and provide for additional definition of PGHS structure-function relationships.

Original languageEnglish
Pages (from-to)499-506
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume227
Issue number2
DOIs
StatePublished - Oct 14 1996

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