TY - JOUR
T1 - Molecular cloning, characterization and mRNA expression of selenium-binding protein in abalone (Haliotis discus hannai Ino)
T2 - Response to dietary selenium, iron and zinc
AU - Wu, Cheng Long
AU - Zhang, Wen Bing
AU - Mai, Kang Sen
AU - Liang, Xu Fang
AU - Xu, Wei
AU - Wang, Jia
AU - Ma, Hong Ming
N1 - Funding Information:
This research was supported financially by grants from the National Natural Science Foundation of China (No. 30972262 and No. 30671630 ) and the National High Technology Research (No. 2007AA09Z437 ). Thanks are also due to Dr. Min Wan and Dr. Qiang Gao for revising this manuscript, and Ms. Qi-yong Chen, Xiao-li Zhong and Bao-juan Liu for their assistance in this work.
PY - 2010/7
Y1 - 2010/7
N2 - Selenium-binding protein (SEBP) is believed to play crucial role in controlling the oxidation/reduction in the physiological processes. In this study, the cDNA of selenium-binding protein from abalone Haliotis discus hannai Ino (HdhSEBP) was cloned by homology cloning and rapid amplification of cDNA ends (RACE) technique. The full length of HdhSEBP cDNA was 2071bp, consisting of a 5' untranslated region (UTR) of 55bp, a 3' UTR of 522bp, and an open reading frame (ORF) of 1494bp. The deduced protein has 497 amino acid residues with a calculated molecular mass of 55.6kDa and a predicted isoelectric point of 5.47. BLAST analysis reveals that HdhSEBP shares high identities with other known SEBPs from mammal, bird, fish and mollusk, etc. The mRNA expression patterns of HdhSEBP in hepatopancreas and haemocytes were measured by real-time PCR in abalone fed with nine different diets containing graded levels of selenium (0, 1 and 50mgKg-1), iron (0, 65 and 1300mgKg-1) and zinc (0, 35 and 700mgKg-1) for 20 weeks, respectively. The results showed that the expression of the HdhSEBP mRNA increased and reached the maximum at optimal dietary selenium (1mgKg-1), iron (65mgKg-1) and zinc (35 mg Kg-1), respectively. Deficient or excessive level of dietary selenium, iron or zinc, respectively, leaded to significant depression of HdhSEBP mRNA. It is concluded that the expression levels of HdhSEBP are affected by dietary selenium, iron or zinc.
AB - Selenium-binding protein (SEBP) is believed to play crucial role in controlling the oxidation/reduction in the physiological processes. In this study, the cDNA of selenium-binding protein from abalone Haliotis discus hannai Ino (HdhSEBP) was cloned by homology cloning and rapid amplification of cDNA ends (RACE) technique. The full length of HdhSEBP cDNA was 2071bp, consisting of a 5' untranslated region (UTR) of 55bp, a 3' UTR of 522bp, and an open reading frame (ORF) of 1494bp. The deduced protein has 497 amino acid residues with a calculated molecular mass of 55.6kDa and a predicted isoelectric point of 5.47. BLAST analysis reveals that HdhSEBP shares high identities with other known SEBPs from mammal, bird, fish and mollusk, etc. The mRNA expression patterns of HdhSEBP in hepatopancreas and haemocytes were measured by real-time PCR in abalone fed with nine different diets containing graded levels of selenium (0, 1 and 50mgKg-1), iron (0, 65 and 1300mgKg-1) and zinc (0, 35 and 700mgKg-1) for 20 weeks, respectively. The results showed that the expression of the HdhSEBP mRNA increased and reached the maximum at optimal dietary selenium (1mgKg-1), iron (65mgKg-1) and zinc (35 mg Kg-1), respectively. Deficient or excessive level of dietary selenium, iron or zinc, respectively, leaded to significant depression of HdhSEBP mRNA. It is concluded that the expression levels of HdhSEBP are affected by dietary selenium, iron or zinc.
KW - CDNA cloning
KW - Haliotis discus hannai
KW - MRNA expression
KW - Minerals
KW - Selenium-binding protein
UR - http://www.scopus.com/inward/record.url?scp=77955053483&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2010.02.028
DO - 10.1016/j.fsi.2010.02.028
M3 - Article
C2 - 20211737
AN - SCOPUS:77955053483
SN - 1050-4648
VL - 29
SP - 117
EP - 125
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 1
ER -