Molecular cloning and chromosome localization of a putative basolateral Na+-K+-2Cl- cotransporter from mouse inner medullary collecting duct (mIMCD-3) cells

E. Delpire, M. I. Rauchman, D. R. Beier, S. C. Hebert, S. R. Gullans

Research output: Contribution to journalArticlepeer-review

246 Scopus citations

Abstract

Electroneutral Na+-K+-2Cl- cotransporters represent one of the major routes for Cl- movement in epithelia. A secretory form of the cotransporter has been described in the basolateral membrane of a variety of epithelia from fish to mammals. We isolated a putative bumetanide-sensitive Na+-K+-2Cl- cotransporter cDNA, BSC2, from mIMCD-3 cells. Northern analysis indicates that in contrast to BSC1, the recently cloned renal-specific apical isoform of the cotransporter, BSC2 is expressed in secretory epithelia and thus appears to represent the basolateral isoform. Furthermore, BSC2 is also expressed in non-polarized cells, such as red cells and myocytes. Sequence comparison and chromosome localization demonstrate that BSC2 and BSC1 are different genes that diverged before the evolution of vertebrates.

Original languageEnglish
Pages (from-to)25677-25683
Number of pages7
JournalJournal of Biological Chemistry
Volume269
Issue number41
StatePublished - 1994

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