TY - JOUR
T1 - Molecular characterization of two novel C-type lectin-like receptors, one of which is selectively expressed in human dendritic cells
AU - Colonna, Marco
AU - Samaridis, Jacqueline
AU - Angman, Lena
PY - 2000
Y1 - 2000
N2 - We have identified two human C-type lectin-like receptors, CLEC-1 and CLEC-2. Both display a single carbohydrate recognition domain and a cytoplasmic tyrosine-based motif. They are homologous to the NK cell receptors NKG2s and CD94 and also to the oxidized low-density lipoprotein receptor 1. CLEC-1 and CLEC-2 are preferentially transcribed in dendritic cells (DC) and in the liver, respectively. Following transient transfection in COS cells, CLEC-1 is expressed intracellularly, perhaps requiring an associated chain to reach the cell surface. CLEC-2 is expressed on the surface of transfected cells as a protein of approximately 33 kDa. CLEC-1 and CLEC-2 genes map to human chromosome 12, most likely in linkage with the NK gene complex (NKC). Thus, the NKC may encode C-type lectin-like receptors expressed not only in NK cells but also in other cells, and at least one of these is of potential importance in regulating DC function.
AB - We have identified two human C-type lectin-like receptors, CLEC-1 and CLEC-2. Both display a single carbohydrate recognition domain and a cytoplasmic tyrosine-based motif. They are homologous to the NK cell receptors NKG2s and CD94 and also to the oxidized low-density lipoprotein receptor 1. CLEC-1 and CLEC-2 are preferentially transcribed in dendritic cells (DC) and in the liver, respectively. Following transient transfection in COS cells, CLEC-1 is expressed intracellularly, perhaps requiring an associated chain to reach the cell surface. CLEC-2 is expressed on the surface of transfected cells as a protein of approximately 33 kDa. CLEC-1 and CLEC-2 genes map to human chromosome 12, most likely in linkage with the NK gene complex (NKC). Thus, the NKC may encode C-type lectin-like receptors expressed not only in NK cells but also in other cells, and at least one of these is of potential importance in regulating DC function.
KW - Antigen presentation
KW - C-type lectin
KW - Dendritic cell
KW - Liver
KW - Macrophage
KW - Monocyte
UR - http://www.scopus.com/inward/record.url?scp=0033952396&partnerID=8YFLogxK
U2 - 10.1002/1521-4141(200002)30:2<697::AID-IMMU697>3.0.CO;2-M
DO - 10.1002/1521-4141(200002)30:2<697::AID-IMMU697>3.0.CO;2-M
M3 - Article
C2 - 10671229
AN - SCOPUS:0033952396
SN - 0014-2980
VL - 30
SP - 697
EP - 704
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 2
ER -