TY - JOUR
T1 - Molecular aspects of fatty acid transport
T2 - Mutations in the IYTSGTTGXPK motif impair fatty acid transport protein function
AU - Stuhlsatz-Krouper, S. M.
AU - Bennett, N. E.
AU - Schaffer, J. E.
PY - 1999
Y1 - 1999
N2 - The murine fatty acid transport protein (FATP) facilitates uptake of long chain fatty acids (LCFAs) when expressed in mammalian cells. FATP's sequence contains a highly conserved motif, IYTSGTTGXPK, also found in a number of proteins known to interact with ATP. To explore the role of this motif, we independently mutated the central serine (serine 250) and threonine (threonine 252) residues in this motif and assessed the effects of these mutations on FATP function. When expressed in fibroblasts, the FATP mutants demonstrated impaired LCFA import and impaired binding of [α-32P]8-azido-ATP (azido-ATP) compared with wild-type FATP. These results suggest that serine 250 and threonine 252 are critical for FATP function and that the mechanism of action of FATP involves nucleotide binding which is dependent on these residues.
AB - The murine fatty acid transport protein (FATP) facilitates uptake of long chain fatty acids (LCFAs) when expressed in mammalian cells. FATP's sequence contains a highly conserved motif, IYTSGTTGXPK, also found in a number of proteins known to interact with ATP. To explore the role of this motif, we independently mutated the central serine (serine 250) and threonine (threonine 252) residues in this motif and assessed the effects of these mutations on FATP function. When expressed in fibroblasts, the FATP mutants demonstrated impaired LCFA import and impaired binding of [α-32P]8-azido-ATP (azido-ATP) compared with wild-type FATP. These results suggest that serine 250 and threonine 252 are critical for FATP function and that the mechanism of action of FATP involves nucleotide binding which is dependent on these residues.
UR - http://www.scopus.com/inward/record.url?scp=0032771089&partnerID=8YFLogxK
U2 - 10.1016/S0952-3278(99)80001-5
DO - 10.1016/S0952-3278(99)80001-5
M3 - Article
C2 - 10471110
AN - SCOPUS:0032771089
VL - 60
SP - 285
EP - 289
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
SN - 0952-3278
IS - 5-6
ER -