TY - JOUR
T1 - Molecular analysis of Ewing's sarcoma
T2 - Another fusion gene, EWS-E1AF, available for diagnosis
AU - Urano, Fumihiko
AU - Umezawa, Akihiro
AU - Yabe, Hiroki
AU - Hong, Wei
AU - Yoshida, Koichi
AU - Fujinaga, Kei
AU - Hata, Jun Ichi
PY - 1998/7
Y1 - 1998/7
N2 - Ewing's sarcoma, one of the most malignant tumors of children and young adults, expresses specific chimeric genes, e.g. EWS-FLI-1, EWS-ERG, EWS-ETV1 and EWS-FEV. In this paper, we extensively characterized a new fusion gene, EWS-E1AF by means of whole cDNA sequencing, RNA blot analysis, DNA blot analysis and chromosomal analysis, and showed it to be available for the diagnosis of Ewing's sarcoma and to participate in the oncogenesis of Ewing's sarcoma. Furthermore, we conducted a genetic analysis of Ewing family tumors in conjuction with immunohistochemical analysis and ultrastructural analysis. Our results demonstrate some limitations of both genetic analysis and histopathological analysis, and establish the relationship between neurogenic phenotypes and chimera genes.
AB - Ewing's sarcoma, one of the most malignant tumors of children and young adults, expresses specific chimeric genes, e.g. EWS-FLI-1, EWS-ERG, EWS-ETV1 and EWS-FEV. In this paper, we extensively characterized a new fusion gene, EWS-E1AF by means of whole cDNA sequencing, RNA blot analysis, DNA blot analysis and chromosomal analysis, and showed it to be available for the diagnosis of Ewing's sarcoma and to participate in the oncogenesis of Ewing's sarcoma. Furthermore, we conducted a genetic analysis of Ewing family tumors in conjuction with immunohistochemical analysis and ultrastructural analysis. Our results demonstrate some limitations of both genetic analysis and histopathological analysis, and establish the relationship between neurogenic phenotypes and chimera genes.
KW - E1AF
KW - Ets
KW - Ewing's sarcoma
KW - Molecular diagnosis
KW - RNA binding protein
UR - http://www.scopus.com/inward/record.url?scp=0031663648&partnerID=8YFLogxK
U2 - 10.1111/j.1349-7006.1998.tb03274.x
DO - 10.1111/j.1349-7006.1998.tb03274.x
M3 - Article
C2 - 9738976
AN - SCOPUS:0031663648
SN - 0910-5050
VL - 89
SP - 703
EP - 711
JO - Japanese Journal of Cancer Research
JF - Japanese Journal of Cancer Research
IS - 7
ER -